Micropatterned surfaces with cell adhesive areas, delimited by protein repellent microstructures, are in high demand for its potential use as relevant biological assays. This is not only because such surfaces allow directing cell growth in a spatially localized and restricted manner, but also because they can be used to elucidate basic cell growth and orientation mechanisms. Here, it is presented a laser-assisted micropatterning technique to fabricate large area microstructures of poly (ethylene glycol) hydrogel onto a cell adhesive surface: a biofunctional maleic anhydride copolymer. By varying photoinitiator, laser intensity, copolymer as well as the hydrogel layer thickness, the optimum conditions to produce high quality features were found. The suitability of these micropatterned substrates for bioassay applications was proved by cell adhesion studies. The introduced procedure could be used to prepare a broad range of microarrays for certain bioanalytical approaches and to create different types of biofunctional surfaces.
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