AI Article Synopsis
- There is a growing demand for affordable and heat-stable PCR reagents for diagnostic testing in low-resource areas.
- Researchers evaluated the thermostability of commercial PCR master mixes, finding that some performed well at 20°C, 40°C, and 45°C for various durations.
- None of the mixes lasted more than 1 month at 45°C, highlighting challenges for clinical diagnostics in regions where maintaining a cold chain is tough.
Article Abstract
There is an increasing need for affordable, thermostable PCR reagents that can be used for diagnostic testing in resource limited settings. The development of point-of-care devices in such settings is highly dependent on the availability and efficacy of thermostable reagents. Here, we assess the thermostability of commercial, intercalating dye-based real-time PCR master mixes. We show that several of these master mixes have thermostability and robust performance at 20°C, 40°C, and 45°C for 6, 4 and 2 weeks, respectively. However, none of the master mixes that we evaluated was able to withstand more than 1 month at 45°C incubation. Our findings have implications for clinical diagnostics in the developing world where cold-chain delivery of diagnostic assays is difficult to guarantee.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.yexmp.2012.05.002 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!