AI Article Synopsis
- GVHD is a common and challenging complication in stem cell transplants, often studied using mouse models to better understand its complex mechanisms and treatments.
- This study details the histological features of GVHD-related fibrosis in specific mice models after human cell injection, highlighting unique tissue infiltration and fibrosis near blood vessels.
- The research combines immunohistochemistry and mRNA analysis to show how both donor and host cytokines interact, contributing to fibrosis development in the affected organs.
Article Abstract
Graft-versus-host disease (GVHD) remains a frequently occurring and difficult-to-treat complication in human allogeneic stem cell transplantation. Murine transplantation models are often used to study and understand the complex pathogenesis of GVHD and to explore new treatment strategies. Although GVHD kinetics may differ in murine and human models, adequate models are essential for identification of the crucial factors responsible for the major pathology in GVHD. We present a detailed description of the specific histological features of a graft-versus-host-induced fibrotic response in xenogeneic RAG2(-/-)γc(-/-) mice after total body irradiation and injection with human peripheral blood mononuclear cells. We describe the full morphological features of this reaction, including a detailed analysis of the specific tissue infiltration patterns of the human peripheral blood mononuclear cells. Our data show the development of fibrosis, predominantly near blood vessels, and reveal different cell populations and specific cell migration patterns in the affected organs. The combination of immunohistochemical cell characterization and mRNA expression analysis of both human (donor)- and murine (host)-derived cytokines reveals an interaction between host tissues and donor-derived cells in an entangled cytokine profile, in which both donor- and host-derived cytokines contribute to the formation of fibrosis.
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| http://dx.doi.org/10.1016/j.bbmt.2012.05.002 | DOI Listing |
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