AI Article Synopsis
- The study investigates the metabolic profile of human umbilical mesenchymal stem cells (HUMSC) and their differentiation into fat cells (adipocytes) using NMR spectroscopy.
- HUMSC were treated with specific compounds over two weeks to induce fat cell formation, and the differentiation was confirmed through staining and gene expression techniques.
- The results showed distinct metabolic differences between HUMSC and adipocytes, identifying key metabolites and suggesting that NMR spectroscopy could effectively monitor stem cell differentiation.
Article Abstract
Aim: To study the metabolic profile of human umbilical mesenchymal stem cells (HUMSC) and adipogenic differentiation by nuclear magnetic resonance (NMR) spectroscopy.
Methods: HUMSC isolated from human umbilical cord stroma were induced to adipocytes over 2 wk by adding dexamethasone, 3-isobutyl-1-methylxanthine, indomethacin, and insulin to the culture medium. Adipogenic differentiation was confirmed by Red O staining and transcription-polymerase chain reaction. Perchloric acid extracts of the HUMSCs and adipocytes (about 7 × 10(6)) were characterized for metabolites by using in vitro high resolution 9.4T NMR spectroscopy.
Results: Several major metabolites, such as: choline, creatine, glutamate and myo-inositol, acetate, and some fatty acids/triglycerides, were observed in the MR spectroscopic pattern of HUMSCs and their adipogenic differentiation. HUMSCs are characterized by an unusually low number of NMR-detectable metabolites, high choline, acetate, glutamate and creatine content. However, the metabolic profiles of adipogenic differentiation demonstrated considerably higher methionine and fatty acids, and non-detectable creatine.
Conclusion: The biomarkers of HUMSCS and adipocytes were obtained and assigned. NMR spectroscopy will be a promising tool for monitoring stem cell differentiation.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3348957 | PMC |
| http://dx.doi.org/10.4252/wjsc.v4.i4.21 | DOI Listing |
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