Morphometric analysis of the optic nerve in experimental hydrocephalus-induced rats.

Purpose: It was the aim of this study to investigate changes caused to the optic nerve of rats submitted to experimental hydrocephalus through morphometric analysis.

Method: At postnatal day 7, the rats underwent injection of kaolin into the cisterna magna, were sacrificed at postnatal day 14, 21 or 28, and the right optic nerves were dissected. We analyzed the area, minor diameter, densities of oligodendrocytes and astrocytes, total and damaged fibers as well as the relationship between damaged and total fibers.

Results: At postnatal day 14, there was a reduction in the density of astrocytes and damaged fibers when compared to the controls. At postnatal day 21, the area and the minor diameter were reduced compared to the controls, and the densities of oligodendrocytes and damaged fibers were increased compared to the controls. At postnatal day 28, there was a reduction in the area and the minor diameter and an increase in the densities of oligodendrocytes, astrocytes and damaged fibers when compared to controls.

Conclusion: The optic nerve of rats submitted to experimental hydrocephalus suffers morphometric changes.