Beef is one of the leading sources of protein, B vitamins, iron, and zinc in human food. Beef palatability is based on three general criteria: tenderness, juiciness, and flavor, of which tenderness is thought to be the most important factor. In this study, we found that beef tenderness, measured by the Warner-Bratzler shear force (WBSF), was dramatically increased by acute stress. Microarray analysis and qPCR identified a variety of genes that were differentially expressed. Pathway analysis showed that these genes were involved in immune response and regulation of metabolism process as activators or repressors. Further analysis identified that these changes may be related with CpG methylation of several genes. Therefore, the results from this study provide an enhanced understanding of the mechanisms that genetic and epigenetic regulations control meat quality and beef tenderness.
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http://dx.doi.org/10.1155/2012/756284 | DOI Listing |
Food Chem
January 2025
Lab of Meat Processing and Quality Control, College of Food Science and Engineering, Shandong Agricultural University, Tai'an, Shandong 271018, PR China. Electronic address:
This study investigated the effect of the inhibition of the activity of Ca-independent Phospholipase A (iPLA) of Peroxiredoxin 6 (Prdx6) on beef tenderization in the early post-mortem period. Longissimus lumborum (LL) were incubated with or without the inhibitor of iPLA activity of Prdx6 (MJ33) for 1, 6, 12, 24, or 36 h, followed by incubation with or without the HO. iPLA activity, troponin T and desmin, Ca concentration, calpain-1, caspases, apoptosis rate, and cell morphology were examined.
View Article and Find Full Text PDFFood Chem X
January 2025
Hubei Key Laboratory of Edible Wild Plants Conservation & Utilization, College of Life Sciences, Hubei Normal University, Huangshi 435002, China.
The study aimed to assess the oxidative modification behavior of bovine myofibrillar proteins (MPs) cysteines (Cys) by hydroxyl radical (·OH) through the construction of an in vitr Fenton reaction system. The ·OH generated by the Fenton reaction induced large-scale oxidative modification of Cys, and redox proteomics identified a total of 1192 differential oxidation sites (Dos), 59 Dos were located in the MPs structure. The Cys of actin (17 Dos), myosin/myomesin (16 Dos), tenascin (12 Dos) and sarcomere (10 Dos) in the MPs structure showed active oxidative modification behavior towards ·OH, especially with the "-C-X-X-X-X-W-" structure amino acid sequence showed high sensitivity.
View Article and Find Full Text PDFFood Chem
January 2025
College of Life Science, Yantai University, No. 30 Qingquan Road, Yantai 264005, Shandong, China. Electronic address:
The impacts of various aging techniques on meat quality and metabolism alterations over time were investigated. Meat tenderness improved with aging, whereas prolonged aging negatively impacted color and oxidative stability. Dry-aging (DA) group exhibited significantly higher (P < 0.
View Article and Find Full Text PDFAnimal
December 2024
School of Agriculture and Food Science, University College Dublin, Belfield, Dublin 4, Ireland.
Exploitation of compensatory growth (CG) is a widely practised management strategy in beef production, especially under pastoral conditions due to its potential to reduce feed costs. The aim of this experiment was to evaluate the effect of nutritional restriction during backgrounding in Angus steers slaughtered at either similar age and/or similar BW on feed efficiency, body composition, carcass characteristics and meat quality attributes under either a forage or feedlot-based finishing diet. Eighty steers (BW: 444 ± 39 kg, age: 18 ± 1 months) were blocked and randomly assigned within block to either an optimal (0.
View Article and Find Full Text PDFMeat Sci
January 2025
Department of Animal and Food Sciences, Texas Tech University, Lubbock, TX 79409, USA. Electronic address:
Steak samples were collected from the longissimus lumborum muscles of beef carcasses (Canada AA, n = 1505; Canada AAA, n = 1363) over a 3-year period. Steaks were aged for 14 d, and tenderness was determined by slice shear force (SSF). Metabolomic profiling of beef samples was performed using rapid evaporative ionization mass spectrometry (REIMS) (N = 2853).
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