In-vitro culture studies for callus and root generation of boerhaavia diffusa linn.

Leaf and stem explants excised from young plant of B.diffusa  were cultured on Murashige and Skoog (MS) medium containing agar (0.8%), sucrose (2.5%) and varied concentration of Indole butyric acid (IBA), Napthyl acetic acid (NAA), 2,4 - dichloro phenoxy acetic acid (2,4-D) and Picrolam. Leaf explants has given better response for both rooting and callus formation. IBA in a concentration of 5μM has shown maximum regeneration (69.7%) with induction period of 7 days. The developed roots were similar to that of naturally grown roots with little anatomical changes. For callus formation 20μM 2, 4-D has given maximum amount and percentage response 979.5% with an induction period of 8 days. Picrolam (10μM) has shown 36.6% response and the average weight of callus was less as compared with 2,4-D. The callus obtained was friable and opaque in nature.