A simple spectrophotometric method is reported her for the estimation of reserpine in polyherbal formulations. Estimation is based on the reaction with3-methylbenzolinone-2hydrazone (MBTH) reagent in presence of cerric ammonium sulphate to yield a violet coloured chromogen, which exhibits and absorption maxima at 580 nm. The chromogen is stable for 10 minutes.

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3331173PMC

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