Aim: To detect the differentiation effects of retinal cells or extracts on bone marrow-derived mesenchymal stem cells (BMSC).
Methods: Human fetal BMSC were previously labelled by carboxyfluorescein succinimidyl ester (CFSE), and co-cultured with retinal pigment epithelial (RPE) cells which were pre-treated with ultraviolet irradiation at a ratio of 1:1 to induce the differentiation of BMSC for up to 14 days. In some assays, a retinal extract of bovine retinal extract (BRE) was added to detect the potential effects of retinal component on the differentiation of BMSC. In addition, Neuron-specific enolase (NSE), Nestin and Glial fibrillary acidic protein (GFAP) immunostaining were performed to determine the characteristics of BMSC.
Results: The results indicated that by co-cultured with RPE cells, fetal BMSC were differentiated into neural-like cells expressing special neuronal markers Nestin, GFAP and NSE. And the expression of these markers was obviously increased by BRE.
Conclusion: Retina derived cells and extracts can induce the differentiation of BMSC into neural-like cells.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3340659 | PMC |
| http://dx.doi.org/10.3980/j.issn.2222-3959.2010.01.06 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Tuft cells transdifferentiate to neural-like progenitor cells in the progression of pancreatic cancer.
Dev Cell
December 2024
Department of Surgery, Henry Ford Health, Detroit, MI, USA; Department of Pharmacology and Toxicology, Michigan State University, Lansing, MI, USA. Electronic address:
Pancreatic ductal adenocarcinoma (PDA) is partly initiated through the transdifferentiation of acinar cells to metaplasia, which progresses to neoplasia and cancer. Tuft cells (TCs) are chemosensory cells not found in the normal pancreas but arise in cancer precursor lesions and diminish during progression to carcinoma. These metaplastic TCs (mTCs) suppress tumor progression through communication with the tumor microenvironment, but their fate during progression is unknown.
View Article and Find Full Text PDFFrom British Associationism to the Hippocampal Cognitive Map: A Personal View From a Ringside Seat at the Cognitive/PDP Revolution.
Hippocampus
January 2025
Department of Psychology, Bowling Green State University, Bowling Green, Ohio, USA.
The mandate for this special issue of Hippocampus was to provide a few examples of one's own work in a relatively personal context. Accordingly, I will discuss some of my own work here, but will also provide a broader arc of ideas and discoveries within which the efforts of myself and many others have taken place. This history begins with the associationists, who proposed that the human mind could be understood, in part, as a compounding of simple associations between contiguously occurring items and events.
View Article and Find Full Text PDFCharacterization of cell states in biliary tract cancers identifies mechanisms of therapeutic resistance in a phase II trial of DKN-01/nivolumab.
medRxiv
October 2024
Department of Medicine, Massachusetts General Hospital, Harvard Medical School, Boston, MA.
Article Synopsis
- Biliary tract cancers show strong resistance to treatments, and effective therapies for these advanced cases are currently limited.
- A clinical trial combined DKN-01 and nivolumab to see if they could help patients with advanced biliary tract cancer, but no positive results were found.
- Researchers analyzed tissue samples to identify different cell types that contribute to the cancer's resistance, discovering new immune and malignant cell states that could inform future treatment strategies.
Varicella-zoster virus recapitulates its immune evasive behaviour in matured hiPSC-derived neurospheroids.
Front Immunol
October 2024
Laboratory of Experimental Hematology (LEH), Vaccine and Infectious Disease Institute (Vaxinfectio), University of Antwerp, Antwerp, Belgium.
Article Synopsis
- * The study analyzed how human neural-like cells (neurospheroids) respond to VZV infection compared to Sendai virus (SeV), finding that SeV triggers a strong immune response while VZV appears to evade detection.
- * The research indicates that VZV not only avoids activating the immune system but also disrupts cellular integrity and prompts stress response mechanisms in the long term.
Nucleoporin 153 deficiency in adult neural stem cells defines a pathological protein-network signature and defective neurogenesis in a mouse model of AD.
Stem Cell Res Ther
September 2024
Department of Neuroscience, Università Cattolica del Sacro Cuore, Rome, 00168, Italy.
Background: Reduction of adult hippocampal neurogenesis is an early critical event in Alzheimer's disease (AD), contributing to progressive memory loss and cognitive decline. Reduced levels of the nucleoporin 153 (Nup153), a key epigenetic regulator of NSC stemness, characterize the neural stem cells isolated from a mouse model of AD (3×Tg) (AD-NSCs) and determine their altered plasticity and gene expression.
Methods: Nup153-regulated mechanisms contributing to NSC function were investigated: (1) in cultured NSCs isolated from AD and wild type (WT) mice by proteomics; (2) in vivo by lentiviral-mediated delivery of Nup153 or GFP in the hippocampus of AD and control mice analyzing neurogenesis and cognitive function; (3) in human iPSC-derived brain organoids obtained from AD patients and control subjects as a model of neurodevelopment.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!