AI Article Synopsis
- Preclinical trials showed that mesenchymal stromal cells (MSCs) can enhance recovery after stroke, mainly due to their immune system-modulating abilities.
- A study was conducted where MSCs were injected into mice following a stroke to examine their effect on stroke-related immune system changes.
- The results indicated that while MSCs have immunosuppressive properties in the lab, they did not significantly affect immune dysfunction or inflammatory cytokine levels in the mice post-stroke, suggesting they are safe for use in clinical trials.
Article Abstract
Preclinical trials confirmed the potential of mesenchymal stromal cells (MSCs) to improve functional recovery after experimental stroke. Beneficial effects of MSCs are often attributed to their immunosuppressive/immunomodulatory functions. Surprisingly, the influence of MSCs on the immune system after stroke is poorly understood, but requires special consideration because cerebral ischemia is associated with stroke-induced immunodepression that predisposes to bacterial infections with increased mortality. In this study, we intravenously transplanted syngeneic murine bone marrow-derived MSCs (mMSCs) into C57BL/6 mice at 6 hours after transient middle cerebral artery occlusion (MCAo; 60 minutes) to investigate the impact of MSCs on stroke-induced immunodepression. Transplantation of syngeneic splenocytes or phosphate-buffered saline (PBS) served as controls. An immune status was determined by flow cytometry on days 3 and 14 after MCAo, which did not reveal any negative effects of cell transplantation on stroke-induced immunodepression. Although our mMSCs were found to exert immunosuppressive effects in vitro, stroke-mediated immune cell dysfunction was not altered by mMSCs in ex-vivo stimulation assays with lipopolysaccharide or concanavalin A. Moreover, systemic inflammatory cytokine levels (interleukin-6, tumor necrosis factorα, interferonγ, monocyte chemoattractant protein-1) remained unchanged in the sera of mice after cerebral ischemia and cell transplantation. These results reduce safety concerns about MSC administration in ongoing clinical stroke trials.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3421097 | PMC |
| http://dx.doi.org/10.1038/jcbfm.2012.55 | DOI Listing |
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