Pseudomonas aeruginosa is a common, free-living, Gram-negative bacterium that can cause significant disease as an opportunistic pathogen. Rapid growth, facile genetics, and a large suite of virulence-related phenotypes make P. aeruginosa a common model organism to study Gram-negative opportunistic pathogens and basic microbiology. This unit describes the basic laboratory growth and maintenance of P. aeruginosa.
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http://dx.doi.org/10.1002/9780471729259.mc06e01s25 | DOI Listing |
J Environ Manage
January 2025
Department of Chemical & Biomolecular Engineering, Institute for Corrosion and Multiphase Technology, Ohio University, Athens, 45701, USA; Department of Biological Sciences, Molecular & Cellular Biology Program, Ohio University, Athens, OH, 45701, USA. Electronic address:
Biofilms can cause biofouling, water quality deterioration, and transmission of infectious diseases. They are also responsible for microbiologically influenced corrosion (MIC) which can cause leaks, resulting in environmental disasters. A new disposable biofilm/MIC test kit was demonstrated to distinguish abiotic corrosion of carbon steel from MIC.
View Article and Find Full Text PDFAdv Sci (Weinh)
January 2025
Department of Clinical Laboratory Medicine, Guangdong Provincial People's Hospital (Guangdong Academy of Medical Sciences), Southern Medical University, Guangzhou, Guangdong, 510000, China.
Immunochromatographic assays (ICAs) provide simple and rapid strategies for bacterial diagnosis but still suffer from the problems of low sensitivity and high dependency on paired antibodies. Herein, the broad-spectrum capture and detection capability of the antibody-free electropositive nanoprobe are clarified for bacteria for the first time and an ultrasensitive fluorescent ICA platform is constructed for the simultaneous diagnosis of multiple pathogens. A magnetic multilayer quantum dot nanocomposite with an amino-embedded SiO shell (MagMQD@Si) is designed to enrich bacteria from solutions effectively, offer high luminescence, and reduce background signals on test strips, thus greatly improving the sensitivity and stability of ICA technique for pathogen.
View Article and Find Full Text PDFACS Appl Mater Interfaces
January 2025
School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou 510006, China.
() infections are increasingly challenging due to their propensity to form biofilms and low outer membrane permeability, especially in chronically infected patients with thick mucus. exhibits multiple drug resistance mechanisms, making it one of the most significant global public health threats. In this study, we found that moxifloxacin (MXC) and antibacterial peptides (ε-poly-l-lysine, ε-PLL) exhibited a synergistic effect against multidrug-resistant (MDR-).
View Article and Find Full Text PDFMacromol Rapid Commun
January 2025
UCL School of Pharmacy, University College London, 29-39 Brunswick Square, London, WC1N 1AX, UK.
Antimicrobial resistance poses a growing threat to public health globally. Multidrug resistant Pseudomonas (P.) aeruginosa is detected in many infected wounds and is very challenging to treat with antibiotics.
View Article and Find Full Text PDFJ Adv Prosthodont
December 2024
Department Prosthetic Dental Sciences, College of Dentistry, Jouf University, Jouf, Saudia Arabia.
Purpose: This study assessed the microgap width and adhesion of three bacterial species in four dental implants with different interlocks under four screwing torques.
Materials And Methods: Ten samples of four implant systems with various interlockings, including full-hexagonal (FHI), cylindrical-conical trilobe-index (TLI), Morse-taper with octagon terminal index (OI), and hexagonal interlock (slip-fit) (HI-SF), were used. The abutments were screwed to the fixtures under torques of 10, 20, 30, and 40 Ncm.
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