Microtubules in spindles are too dense to resolve by light microscopy, even with super-resolution methods. Using a new method based on laser-ablation techniques, Brugués et al. present the first quantitative characterization of the vertebrate meiotic spindle and propose an assembly mechanism for building this architecture.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.cell.2012.04.006 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
The plantaris muscle is spindle-shaped in the posterior compartment of the leg. It is distinguished for its small muscle belly and an exceptionally long tendon. It presents with great variability in its origin and insertion when present.
View Article and Find Full Text PDFS100-positive stroma in salivary gland basal cell adenomas: a neoplastic component with CTNNB1 mutations.
Virchows Arch
December 2024
Division of Translational Oncoimmunology, Aichi Cancer Center Research Institute, Nagoya, Japan.
Basal cell adenomas (BCAs) are benign epithelial tumors of the salivary gland, characterized by the proliferation of basaloid and luminal cells. In addition, a distinctive spindle cell stroma, that is immunohistochemically-positive for S100, is often observed in BCAs. Based on the ultrastructural findings, the S100-positive stroma was presumed to originate from neoplastic myoepithelial cells.
View Article and Find Full Text PDFEmployment of a Newly Defined In Vitro Fertilization Protocol to Determine the Cytoskeletal Machinery, DNA Damage, and Subsequent DNA Repair Resulting from Endocrine Disruption by Hexavalent Chromium in Rat Metaphase II Oocytes.
Curr Protoc
December 2024
Department of Integrative Biosciences, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, Texas.
These protocols describe a detailed method to determine the DNA damage and F-actin and microtubule defects of metaphase II oocytes caused by hexavalent chromium, Cr(VI), an endocrine disrupting chemical (EDC). The protocol provides systematic steps to determine protein expression encoded by pluripotency proteins such as Oct4, Nanog, and Cdx2 during early embryonic development. Occupational or environmental exposure to EDCs has significantly increased infertility in both men and women.
View Article and Find Full Text PDFManipulation of Spindle Position Using Magnetic Tweezers in Sea Urchin Embryos.
Methods Mol Biol
December 2024
Université Paris Cité, CNRS, Institut Jacques Monod, Paris, France.
Article Synopsis
- The proper positioning and orientation of the mitotic spindle during cell division is essential for early embryo development, maintaining tissue structure, and regulating stem cell function.
- Errors in spindle positioning can lead to tissue disorders and contribute to tumor development in stem cells.
- The study presents a technique using in vivo magnetic tweezers to control spindle position and orientation in sea urchin embryos, allowing researchers to study the effects of spindle manipulation on embryo development and potentially apply this method to other cell types.
SKA1/2/3 is a prognostic and predictive biomarker in esophageal adenocarcinoma and squamous cell carcinoma.
BMC Cancer
November 2024
Medical Research Center, Affiliated Hospital of Jining Medical University, Jining Medical University, Jining, Shandong Province, 272029, P. R. China.
Background: Esophageal carcinoma (ESCA) ranks among the most prevalent malignant tumors globally. Despite significant advancements in treatment options and improved patient outcomes, the 5-year survival rate remains unsatisfactory. The spindle and kinetochore associated complex subunit 1/2/3 (SKA1/2/3) attached to the kinetochore (KT) in the metaphase of mitosis are implicated in the occurrence and development of various tumors.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!