AI Article Synopsis
- The study discovered that norcantharidin has anti-angiogenesis effects on human colon cancer, which was previously unexpected.
- Researchers used a tumor xenograft model in nude mice and confirmed that norcantharidin (5 or 15 mg/kg) inhibits angiogenesis in vivo.
- In vitro experiments showed that norcantharidin reduced the migration, adhesion, and tube formation abilities of human umbilical vein endothelial cells (HUVECs), impacting the expression of key proteins involved in angiogenesis.
Article Abstract
The present study was based on the unexpected discovery that norcantharidin exerted anti-angiogenesis activity when effects on growth of human colon cancer were studied. The aim was to further verify this finding and explore possible mechanisms using a tumor xenograft model in nude mice. We confirmed that norcantharidin (5 or 15 mg/kg) could inhibit angiogenesis of human colon cancer in vivo. In vitro, crossing river assay, cell adhesion assay and tube formation assay indicated that NCTD could reduce the migration, adhesion and vascular network tube formation ability of HUVECs. At the same time, the expression levels of VEGF and VEGFR-2 proteins which play important roles in angiogenesis were reduced as examined by western blotting analysis. Taken together, the results firstly showed NCTD could inhibit angiogenesis of human colon cancer in vivo, probably associated with effects on migration, adhesion and vascular network tube formation of HUVECs and expression levels of VEGF and VEGFR-2 proteins.
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| http://dx.doi.org/10.7314/apjcp.2012.13.2.499 | DOI Listing |
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