AI Article Synopsis
- The study highlights an increased detection of low-level leukemic blasts in the cerebrospinal fluid (CSF) of pediatric acute lymphoblastic leukemia (ALL) patients after switching from the Shandon CytoSpin to the Wescor Cytopro cytocentrifuge.
- The Wescor Cytopro showed a significant increase, 5- to 9-fold, in the number of cells on slides compared to the Shandon CytoSpin and identified leukemic blasts in two cases that were missed by the older machine.
- These findings indicate that the detection of leukemic blasts is highly dependent on the cytocentrifuge used, and while newer machines may uncover previously undetected blasts, the clinical implications of this increased sensitivity are still
Article Abstract
Staging and monitoring of pediatric acute lymphoblastic leukemia (ALL) includes examination of the cerebrospinal fluid (CSF). At our institution, we noted an increased incidence of low-level leukemic blasts in CSF samples from patients with ALL. This increase coincided with a conversion from the Shandon CytoSpin 4 (Thermo Fisher Scientific, Waltham, MA) to the Wescor Cytopro Rotor AC-060 (Wescor, Logan, UT). This study directly compared these 2 machines using patient samples and known concentrations of cultured leukemia cells. With patient samples, the Wescor Cytopro led to a 5- to 9-fold increase in the number of cells on a slide compared with the Shandon CytoSpin; furthermore, leukemic blasts were detected only with the Wescor Cytopro in 2 cases. Similar findings were observed using cultured leukemia cells. Thus, the detection of blasts in CSF is highly instrument-dependent. The newer, more sensitive cytocentrifuge machines identify blasts that were previously missed by older machines, but the clinical significance remains under investigation.
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| http://dx.doi.org/10.1309/AJCPP4HQ2FXQEZIL | DOI Listing |
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Article Synopsis
- The study highlights an increased detection of low-level leukemic blasts in the cerebrospinal fluid (CSF) of pediatric acute lymphoblastic leukemia (ALL) patients after switching from the Shandon CytoSpin to the Wescor Cytopro cytocentrifuge.
- The Wescor Cytopro showed a significant increase, 5- to 9-fold, in the number of cells on slides compared to the Shandon CytoSpin and identified leukemic blasts in two cases that were missed by the older machine.
- These findings indicate that the detection of leukemic blasts is highly dependent on the cytocentrifuge used, and while newer machines may uncover previously undetected blasts, the clinical implications of this increased sensitivity are still
The patented Cytopro system design makes significant advances in the convenience and reliability of cytocentrifugation. The chamber and pad are designed to facilitate prewetting and to better control fluid flow rates. The sealed rotor is durable, convenient, and reliable with significant patented features impacting sample quality and convenience of operation.
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