Brevibacterium yomogidense sp. nov., isolated from a soil conditioner made from poultry manure.

A novel Gram-stain-positive rod-shaped actinobacterium was isolated from a soil conditioner made from poultry manure. The isolate, designated strain MN-6-a(T), contained anteiso-C(15 : 0) and anteiso-C(17 : 0) as the major fatty acids, and MK-7(H(2)) and MK-8(H(2)) as the major menaquinones. Phosphatidylglycerol was a major polar lipid. The G+C content of the genomic DNA was 67.4 mol%. Phylogenetic analysis showed that strain MN-6-a(T) was closely related to Brevibacterium salitolerans TRM 415(T) with 97.1 % 16S rRNA gene sequence similarity. DNA-DNA hybridization showed that strain MN-6-a(T) had 10.2 % genomic relatedness with B. salitolerans TRM 415(T). On the basis of phenotypic, phylogenetic and chemotaxonomic data obtained in this study, strain MN-6-a(T) represents a novel species of the genus Brevibacterium, for which the name Brevibacterium yomogidense sp. nov. is proposed. The type strain is MN-6-a(T) ( = JCM 17779(T) = DSM 24850(T)).