We report a miniaturized resonant/non-resonant multi-fiber raster scanner that is paired with a gradient-index lens assembly to achieve a compact and flexible multifocal multiphoton endoscope capable of longitudinal parallel image acquisition. Multiphoton images are obtained simultaneously at three axial depths, separated by ≥4.8 μm, by incorporating three axially offset double clad optical fibers into the miniaturized scanner. The fabricated endoscope has an outer diameter of 3 mm, a rigid length of 4 cm, and acquires images at 4 frames/s per focal plane, with lateral and axial resolutions for two-photon imaging of 0.8 and 10 μm, respectively.
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http://dx.doi.org/10.1364/OL.37.001349 | DOI Listing |
bioRxiv
February 2023
University of Arizona, Wyant College of Optical Sciences, 1630 E. University Blvd, Tucson, AZ, 85721, USA.
Pancreatic neuroendocrine tumors (PNETs) are a rare but increasingly more prevalent cancer with heterogeneous clinical and pathological presentation. Surgery is the preferred treatment for all hormone-expressing PNETs and any PNET greater than 2 cm, but difficulties arise when tumors are multifocal, metastatic, or small in size due to lack of effective surgical localization. Existing techniques such as intraoperative ultrasound provide poor contrast and resolution, resulting in low sensitivity for such tumors.
View Article and Find Full Text PDFBiophys Chem
April 2022
Centre for Therapeutic Innovation, Cell Biophysics Laboratory, Department of Pharmacy and Pharmacology, Department of Physics, University of Bath, Claverton Down, Bath, United Kingdom; Cell Biophysics Laboratory, Research Centre for Experimental Marine Biology and Biotechnology (PiE) & Instituto Biofisikia (Biophysics Institute) (UPV/ EHU, CSIC), University of the Basque Country, Leioa, Biscay, Spain; Early Phase Trials and Sarcoma, Institut Bergonié, Cours de l'Argonne, Bordeaux, France.
Oncogene dysregulation is a driver of neoplasia development and progression. The use of quantitative molecular imaging to quantify oncogene activation will be crucial in developing companion diagnostics which can identify personalised patient regimens. However, the evaluation of oncogene activation does not necessarily correlate with oncoprotein activation.
View Article and Find Full Text PDFIn this investigation, we propose a strip segmentation phase (SSP) method for a spatial light modulator (SLM) to generate independent multifocal spots when the beam passes through a high numerical aperture (NA) lens. With the SSP method, multifocal spots can be generated with each spot independently, flexibly and uniformly distributed. The performance of the SSP method is first validated with numerical simulation.
View Article and Find Full Text PDFOptica
January 2019
Department of Mechanical Engineering, Massachusetts Institute of Technology, 77 Massachusetts Ave., Cambridge, Massachusetts 02139, USA.
Simultaneous, high-resolution imaging across a large number of synaptic and dendritic sites is critical for understanding how neurons receive and integrate signals. Yet, functional imaging that targets a large number of submicrometer-sized synaptic and dendritic locations poses significant technical challenges. We demonstrate a new parallelized approach to address such questions, increasing the signal-to-noise ratio by an order of magnitude compared to previous approaches.
View Article and Find Full Text PDFTo improve imaging speed, multifocal excitation is widely adopted as a parallel strategy in laser-scanning microscopy. Specifically, axial multifocal microscopy is popular in neuroscience as it enables functional imaging of neurons in multiple depths simultaneously. However, previous phase searching algorithms for axial multi-foci generation generally generate foci of uniform intensities, which cannot compensate the scattering-induced power loss in deep tissue and causes inhomogeneous excitation.
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