To assess the prevalence of Toscana virus (TOSV) infection among healthy residents of Croatia we tested sera from 2016 persons, for IgG antibodies to TOSV, by an enzyme immunoassay. A total of 755 (37.5%) persons had IgG antibodies to TOSV: 53.9%, 33.6% and 6.1% among residents of the islands, coastal area and mainland of Croatia, respectively. Risk factors significantly associated with seropositivity to TOSV were: living on islands (OR, 11.10; 95% CI, 6.02-20.50; p <0.001) or in coastal areas (OR, 6.96; 95% CI, 3.81-12.71; p <0.001) and increase of age (OR, 1.03; 95% CI, 1.02-1.03; p <0.001).
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