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A Single-Step Magnetic Bead-Based Sandwich Immunoassay for the Rapid Detection of Ciguatoxins in Fish Samples.
Chemosphere
January 2025
IRTA, Ctra. Poble Nou km 5.5, 43540 La Ràpita, Spain. Electronic address:
Ciguatera poisoning (CP) is one of the most prevalent non-bacterial seafood-borne illnesses worldwide, caused by the consumption of fish contaminated with ciguatoxins (CTXs). Even though its prevention largely relies on avoidance of certain fishes and geographic regions, the development of rapid and user-friendly bioanalytical methods for effective CP management and surveillance is still necessary. In this work, four simplified strategies of a magnetic bead (MB)-based sandwich immunoassay were proposed for the rapid and highly sensitive detection of CTXs in fish.
View Article and Find Full Text PDFDevelopment of lateral flow immunochromatographic assay with Anti-Pythium insidiosum antibodies for point-of-care testing of vascular pythiosis.
Sci Rep
January 2025
Center of Excellence for Antimicrobial Resistance and Stewardship, Department of Microbiology, Faculty of Medicine, Chulalongkorn University, Bangkok, 10330, Thailand.
The pathogenic oomycete Pythium insidiosum causes a fatal infectious illness known as pythiosis, impacting humans and certain animals in numerous countries in the tropics and subtropics. Delayed diagnosis is a primary factor contributing to the heightened morbidity and mortality associated with the disease. Several new serodiagnostic methods have been developed to improve the identification of pythiosis.
View Article and Find Full Text PDFDisposable and sensitive electrochemical magneto-immunosensor for point-of-care HCV diagnostics: Targeting HCVcAg, the active viremia biomarker, in patient samples.
Biosens Bioelectron
December 2024
Centre for Biomedicine, Hull York Medical School, University of Hull, Hull HU6 7RX, United Kingdom. Electronic address:
Early detection of hepatitis C virus (HCV) infection is crucial for eliminating this silent killer, especially in resource-limited settings. HCV core antigen (HCVcAg) represents a promising alternative to the current "gold standard" HCV RNA assays as an active viremia biomarker. Herein, a highly sensitive electrochemical magneto-immunosensor for the HCVcAg was developed.
View Article and Find Full Text PDFNanoparticle-mediated light-driven LAMP combined with test strips for sensitive and rapid visual detection of antibiotic resistance genes.
J Hazard Mater
December 2024
State Key Laboratory of Food Science and Resources, Jiangnan University, Wuxi 214122, China; International Joint Laboratory on Food Safety, Institute of Analytical Food Safety, School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, China. Electronic address:
Antibiotic resistance genes (ARGs) are markers of drug-resistant pathogens, monitoring them contributes to prevent resistance to drugs. The detection methods for ARGs including PCR and isothermal amplification are sensitive and selective. However, it may take several hours or cannot be used on spot.
View Article and Find Full Text PDFAn integrated magnetoimpedance biosensor microfluidic magnetic platform for the evaluation of the cardiac marker cTnI.
Anal Methods
January 2025
Microelectronic Research & Development Center, School of Mechatronics Engineering and Automation, Shanghai University, Shanghai 200444, China.
An integrated magnetoimpedance (MI) biosensor microfluidic magnetic platform was proposed for the evaluation of the cardiac marker, cardiac troponin I (cTnI). This bioanalyte evaluation platform mainly comprised three external permanent magnets (PMs), one MI element, two peelable SiO film units and a microfluidic chip (MFC). The MI element was made of micro-electro-mechanical system (MEMS)-based multilayered [Ti (6 nm)/FeNi (100 nm)]/Cu (400 nm)/[Ti (6 nm)/FeNi (100 nm)] thin films and designed as meander structures with closed magnetic flux.
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