Objective: To explore a simple and feasible technique to locate proteins during spermatogenesis.
Methods: Various germ cells and somatic cells were separated by collagenase I and DNase I after the albuginea was removed. The cells were then smeared, dyed, and observed directly under fluorescence and confocal microscopy.
Results: Germ cells at different steps were successfully identified by specific dyestuffs for acrosome and nucleolus.
Conclusion: A simple method for locating proteins during spermatogenesis was successfully developed.
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