Objective: To establish the HPLC fingerprint analysis method for the quality control of acetoacetate extraction of Polygonum orientale.
Methods: Agilent C18 (4.6 mm x 250 mm, 5 microm); DAD detector; Mobile phase: methyl alcohol-water gradient elution at a flow rate of 1.0 mL/min, and at a column temperature of 30 degrees C.
Results: 26 peaks were selected as the common fingerprint peaks. The relative standard deviations for relative retention values were less than 3% in the precision and repeated test.
Conclusion: The repetition, stability and precision of the method is good and it can be used to control the quality of Polygonum orientale.
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