We have explored different strategies for sequencing of major breakpoint (mbr) junctional regions in t(14;18) chromosomal translocations--the most frequent chromosomal abnormality observed in B-cell lymphomas. We demonstrate that coupling of the preparation of single-stranded DNA by asymmetric polymerase chain reaction (PCR) and direct sequencing is the method of choice for the rapid and precise determination of clone-specific bcl-2/JH fusion gene sequences. The rapidity, relative ease, and accuracy of the technique, described for the nucleotide sequence analysis of mbr t(14;18) breakpoints, permits the analysis of a relatively large number of samples and should be considered as part of the clinical evaluation of lymphoma patients. Furthermore, by providing sequence information of clone-specific DNA regions, the procedure should reduce the risk of false-positive results from PCR.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1002/path.1711620205 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Phylogeographic Characterization of Aedes albopictus (Skuse, 1894) in Algeria.
Acta Parasitol
January 2025
Laboratory of Parasitic Eco-Epidemiology and Population Genetics, Pasteur Institute of Algeria, Dely-Brahim, Algiers, Algeria.
Purpose: Aedes albopictus, known as the Asian tiger mosquito, is an extensively studied mosquito species recognized for its rapid global expansion and its capacity to transmit a range of viruses such as dengue, chikungunya, Zika, and yellow fever viruses. In 2010, Ae. albopictus was observed for the first time in Tizi-Ouzou, Algeria, and since then has colonized all the northern part of the country until the semi-arid areas.
View Article and Find Full Text PDFIdentification of PIF1 as a Ferroptosis-Related Prognostic Biomarker Correlated with Immune Infiltration in Hepatocellular Carcinoma.
Appl Biochem Biotechnol
January 2025
Department of General Surgery, Shanxi Provincial Cancer Hospital/Shanxi Hospital Cancer Hospital of Chinese Academy of Medical Sciences, No. 3, Gongye New Street , Xinhualing District, Taiyuan, 030001, China.
Hepatocellular carcinoma (HCC) is a primary liver malignancy characterized by high morbidity and mortality. Recently, ferroptosis has been recognized as an important factor in regulating cell growth in HCC. However, the role of ferroptosis-related genes in HCC remains unclear.
View Article and Find Full Text PDFThe candidate gene SibHLHA regulates anthocyanin-driven purple pigmentation in Sesamum indicum flowers.
Theor Appl Genet
January 2025
Henan Sesame Research Center, Henan Academy of Agricultural Sciences, Zhengzhou, China.
Anthocyanins not only serve as critical pigments determining floral hues but also play essential roles in attracting insects for pollination, feeding animals and mitigating abiotic stress. However, the molecular mechanisms underlying the regulation of flower color in sesame has not yet been reported. In this study, an F population was constructed by crossing 'Ganzhi 9' (purple-flowered) with 'BS377' (white-flowered).
View Article and Find Full Text PDFDiscovery of the Novel HLA-C*01:282 Allele by Next-Generation Sequencing.
HLA
February 2025
Histocompatibility and Immunogenetics Laboratory, St. Paul's Hospital, Saskatoon, Saskatchewan, Canada.
The novel allele HLA-C*01:282 has a single non-synonymous mutation compared to HLA- C*01:02:01:01 in codon 100 in Exon 3.
View Article and Find Full Text PDFReference-Based Standardization Approach Stabilizing Small Batch Risk Prediction via Polygenic Score.
Genet Epidemiol
March 2025
Division of Biomedical Information Analysis, Iwate Tohoku Medical Megabank Organization, Disaster Reconstruction Center, Iwate Medical University, Yahaba, Japan.
The polygenic score (PGS) holds promise for motivating preventive behavioral changes. However, no clinically validated standardization methodology currently exists. Here, we demonstrate the efficacy of a "reference-based" approach for standardization.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!