Objective: To optimize the extraction technology of total flavonoids and improve the extraction ratio of total flavonoids from Selaginella uncinata.
Methods: Using the content of flavonoids as the index determined by UV spectrophotometry, chose single factor experiment to investigate the total flavonoids content influenced by four factor ethanol concentration, extracting time, extraction times and solvent content on the influence of extraction efficiency. And on the basis, L9 (3(4)) orthogonal experiment was carried out.
Results: The ethanol reflux extraction conditions were as tollows: extracted in 60% ethanol (solid-liquid rate at 1: 35) for 3 times and heated for 1.5 hours per time.
Conclusion: The optimum extraction by the orthogonal design method is rational and feasible, provides experimental basis for improving the content of the total flavonoids and further scientific theoretical basis for industrial production of the total flavonoids from Selaginella uncinata.
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