A novel chemiluminescence method coupled with flow injection technique for the determination of ractopamine was developed. It was based on the enhancement of the chemiluminescence by ractopamine derived from the chemiluminescence reaction between luminol and ferricyanide in sodium hydroxide medium. The linear calibration range of the chemiluminescence intensity with respect to the ractopamine concentration covers from 4.0 x 10(-9) - 8.0 x 10(-7) g x mL(-1). The relative standard deviation for ractopamine is 5.6% (n = 11), and the detection limit is 2.5 x 10(-9) g x mL(-1). The method was firstly applied to the determination of ractopamine in biological samples with satisfactory results. The recovery was between 69.3% and 101.3%.
Download full-text PDF |
Source |
|---|
Publication Analysis
Top Keywords
Similar Publications
Nanozyme-Enzyme Cascade Reaction-Enhanced Ratiometric Fluorescence Immunosensing Platform for Sensitive and Accurate Detection of Ractopamine.
J Agric Food Chem
November 2024
National Key Laboratory of Veterinary Public Health and Safety, Beijing Key Laboratory of Detection Technology for Animal Derived Food Safety, College of Veterinary Medicine, China Agricultural University, Beijing 100193, People's Republic of China.
As the most widely used immunoassay, enzyme-linked immunosorbent assay (ELISA) relies on perishable enzymes and usually provides poor sensitivity and stability, limiting its application in detecting trace analytes in harsh environments. Herein, a new ratiometric fluorescence (RF) sensing platform enhanced by a nanozyme-enzyme cascade reaction composed of MnO nanosheets (MnO NSs) and alkaline phosphatase (ALP) was proposed. In this RF platform, the versatile MnO NSs worked as a robust oxidase-like nanozyme to catalyze nonfluorescent Amplex Red (AR) into fluorescent resorufin and as a quencher to quench the fluorescence of carbon dots (CDs).
View Article and Find Full Text PDFA multilevel cooperative attention network of precise quantitative analysis for predicting ractopamine concentration via adaptive weighted feature selection and multichannel feature fusion.
Food Chem
February 2025
National R & D Center for Agro-processing Equipment, College of Engineering, China Agricultural University, Beijing 100083, China.
Surface enhanced Raman spectroscopy (SERS) holds great potential due to its rapid detection and high sensitivity. However, issues such as signal noise, fluctuations, and spectral shifts can negatively impact its performance in detecting ractopamine in pork. Hierarchical Gradient Aware Spectral Network (HGASNet) was proposed to address these issues.
View Article and Find Full Text PDFMicrofluidic Detection Platform for Determination of Ractopamine in Food.
Biosensors (Basel)
September 2024
Department of Engineering Science, National Cheng Kung University, Tainan 70101, Taiwan.
A novel microfluidic ractopamine (RAC) detection platform consisting of a microfluidic RAC chip and a smart analysis device is proposed for the determination of RAC concentration in meat samples. This technology utilizes gold nanoparticles (AuNPs) modified with glutamic acid (GLU) and polyethyleneimine (PEI) to measure RAC concentration in food products. When RAC is present, AuNPs aggregate through hydrogen bonding, causing noticeable changes in their optical properties, which are detected using a self-built UV-visible micro-spectrophotometer.
View Article and Find Full Text PDFArticle Synopsis
- Physiologically Based Pharmacokinetic (PBPK) models can predict how drugs like ractopamine (RAC) behave in organisms, and this study focused on developing a PBPK model specifically for goats after multiple oral doses of RAC.
- The model successfully forecasted the drug's distribution across various tissues, with the lung blood flow volume being the most influential factor affecting plasma, liver, and kidney drug concentrations.
- The research also determined the withdrawal time for RAC, finding that it takes 13 days for the drug to clear from the kidneys, which is crucial for safe drug administration in goats.
High Resolving Power Electrospray Ionization Ion Mobility Spectrometer Based on Fourier Deconvolution Multiplexing.
Anal Chem
October 2024
College of Chemical Engineering, Xiangtan University, Xiangtan 411105, China.
Article Synopsis
- - The resolving power of drift tube ion mobility spectrometry (IMS) relies on several factors, including drift length, voltage, pulse width of the ion gate, and tube pressure.
- - Electrospray ionization (ESI)-IMS offers high sensitivity for analyzing nonvolatile compounds, necessitating high resolving power to distinguish similar structures effectively.
- - The study explores Fourier deconvolution (FD) multiplexing to enhance resolving power and signal-to-noise ratio, demonstrating that a 5 μs ion gate opening can achieve a resolving power of 170 without extending the drift length, successfully separating structurally similar compounds.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!