Dietary potassium (K(+)) restriction and hypokalemia have been reported to change the abundance of most renal Na(+) and K(+) transporters and aquaporin-2 isoform, but results have not been consistent. The aim of this study was to reexamine Na(+), K(+) and H(2)O transporters' pool size regulation in response to removing K(+) from a diet containing 0.74% NaCl, as well as from a diet containing 2% NaCl (as found in American diets) to blunt reducing total diet electrolytes. Sprague-Dawley rats (n = 5-6) were fed for 6 days with one of these diets: 2% KCl, 0.74% NaCl (2K1Na, control chow) compared with 0.03% KCl, 0.74% NaCl (0K1Na); or 2% KCl, 2%NaCl (2K2Na) compared with 0.03% KCl, 2% NaCl (0K2Na, Na(+) replete). In both 0K1Na and 0K2Na there were significant decreases in: 1) plasma [K(+)] (<2.5 mM); 2) urinary K(+) excretion (<5% of control); 3) urine osmolality and plasma [aldosterone], as well as 4) an increase in urine volume and medullary hypertrophy. The 0K2Na group had the lowest [aldosterone] (172.0 ± 17.4 pg/ml) and lower blood pressure (93.2 ± 4.9 vs. 112.0 ± 3.1 mmHg in 2K2Na). Transporter pool size regulation was determined by quantitative immunoblotting of renal cortex and medulla homogenates. The only differences measured in both 0K1Na and 0K2Na groups were a 20-30% decrease in cortical β-ENaC, 30-40% increases in kidney-specific Ste20/SPS1-related proline/alanine-rich kinase, and a 40% increase in medullary sodium pump abundance. The following proteins were not significantly changed in both the 0 K groups: Na(+)/H(+) exchanger isoform 3; Na(+)-K(+)-Cl(-) cotransporter; Na(+)-Cl(-) cotransporter, oxidative stress response kinase-1; renal outer medullary K(+) channel; autosomal recessive hypercholesterolemia; c-Src, aquaporin 2 isoform; or renin. Thus, despite profound hypokalemia and renal K(+) conservation, we did not confirm many of the changes that were previously reported. We predict that changes in transporter distribution and activity are likely more important for conserving K(+) than changes in total abundance.
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http://dx.doi.org/10.1152/ajprenal.00032.2012 | DOI Listing |
Sci Rep
July 2023
Centre of Excellence for Research in Engineering Materials (CEREM), Deanship of Scientific Research, King Saud University, 11421, Riyadh, Saudi Arabia.
Int J Syst Evol Microbiol
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State Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, PR China.
A novel bacterial strain, designated as PHS-Z21, was isolated from the marine sponge collected from PG Dave's Rock, Philippines. Cells of PHS-Z21 are Gram-stain-negative, non-motile, pale-yellow-pigmented, short rods. PHS-Z21 is able to grow at 10-40 ℃ (optimum, 30 ℃), pH 5.
View Article and Find Full Text PDFInt J Environ Res Public Health
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Department of Food Science and Human Nutrition, Michigan State University, East Lansing, MI 48824, USA.
(1) Background: Sleep may be a factor that influences the taste-dietary intake relationship. The effect of sleep on salt taste measures has not been adequately studied, and no standardized methodology has been developed for measuring salt taste preference. (2) Methods: A sweet taste forced-choice paired-comparison test was adapted and validated to determine salt taste preference.
View Article and Find Full Text PDFEnviron Sci Pollut Res Int
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Universidad de Alcalá, Facultad de Ciencias, Departamento de Ciencias de la Vida, Unidad de Ecología, Biological Invasions Research Group, Universidad de Alcalá, Plaza de San Diego S/N, Madrid, 28801, Alcalá de Henares, Spain.
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November 2022
Key Laboratory of Environment Remediation and Ecological Health, Ministry of Education, College of Environmental & Resource Science, Zhejiang University, Hangzhou 310058, China.
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