We developed a quantitative PCR method for tracking the dxnA1 gene, the initial, megaplasmid-borne gene in Sphingomonas wittichii RW1's dibenzo-p-dioxin degradation pathway. We used this method on complex environmental samples and report on growth of S. wittichii RW1 in landfill leachate, thus furnishing a novel tool for monitoring megaplasmid-borne, dioxygenase-encoding genes.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3370553 | PMC |
| http://dx.doi.org/10.1128/AEM.00715-12 | DOI Listing |
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