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Boron uptake in normal melanocytes and melanoma cells and boron biodistribution study in mice bearing B16F10 melanoma for boron neutron capture therapy. | LitMetric

AI Article Synopsis

  • The study focused on understanding how boron (10B) distributes in normal tissues and B16F10 melanoma cells to advance the development of boron neutron capture therapy (BNCT) for melanoma treatment.
  • B16F10 melanoma cells exhibited significantly higher boron uptake compared to normal melanocytes, with peak boron concentration occurring 150 minutes post-application, suggesting a more effective treatment approach for melanoma.
  • The research found that while boron levels in the blood of mice decreased over time, tumor boron concentration continued to rise until reaching its maximum at 150 minutes, supporting the potential of BNCT as a promising option for treating chemoresistant melanoma.

Article Abstract

Information on (10)B distribution in normal tissues is crucial to any further development of boron neutron capture therapy (BNCT). The goal of this study was to investigate the in vitro and in vivo boron biodistribution in B16F10 murine melanoma and normal tissues as a model for human melanoma treatment by a simple and rapid colorimetric method, which was validated by HR-ICP-MS. The B16F10 melanoma cell line showed higher melanin content than human melanocytes, demonstrating a greater potential for boronophenylalanine uptake. The melanocytes showed a moderate viability decrease in the first few minutes after BNCT application, stabilizing after 75 min, whereas the B16F10 melanoma showed the greatest intracellular boron concentration at 150 min after application, indicating a different boron uptake of melanoma cells compared to normal melanocytes. Moreover, at this time, the increase in boron uptake in melanoma cells was approximately 1.6 times higher than that in normal melanocytes. The (10)B concentration in the blood of mice bearing B16F10 melanoma increased until 90 min after BNCT application and then decreased after 120 min, and remained low until the 240th minute. On the other hand, the (10)B concentration in tumors was increased from 90 min and maximal at 150 min after application, thus confirming the in vitro results. Therefore, the present in vitro and in vivo study of (10)B uptake in normal and tumor cells revealed important data that could enable BNCT to be possibly used as a treatment for melanoma, a chemoresistant cancer associated with high mortality.

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Source
http://dx.doi.org/10.1007/s00411-012-0416-yDOI Listing

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