AI Article Synopsis
- - Adenovirus is commonly used in gene therapy and vaccines, with hypervariable regions (HVRs) on its hexon proteins being crucial for how the virus is recognized by the immune system.
- - Genetic engineering of these HVRs can change the virus's antigenic properties and impact its ability to interact with coagulation factor X (FX), which is important for the virus’s effectiveness in liver transduction.
- - Recent experiments showed that certain HVRs (HVR5 and HVR7) have reduced ability to neutralize antibodies compared to others, and that HVRs influence the binding affinity with FX, affecting the overall function and structure of adenovirus hexon proteins.
Article Abstract
Adenovirus is widely used in gene therapy and vaccination as a viral vector, and its hypervariable regions (HVRs) on hexon are the main antigen recognition sites of adenovirus. The modification of this area by genetic engineering will change the antigenic specificity of the virus. In addition, recent studies have demonstrated the importance of coagulation factor X (FX) in adenovirus serotype 5-mediated liver transduction in vivo. The binding site of adenovirus to FX is the HVRs on hexon. By constructing five proteins containing chimeric HVRs from different adenovirus serotypes, we focused on the antigenic specificity and the affinity for FX of these proteins compared with the corresponding viruses. Our data showed that HVR5 and HVR7 had only a part of hexon activity to neutralizing antibodies (NAbs) compared with the complete activity of HVR1-7. Results also demonstrated a differential high-affinity interaction of the HVRs proteins with FX and indicated that HVRs protein had a similar binding ability with corresponding adenovirus serotype. These results highlighted some properties of chimeric HVRs proteins and revealed the influence on the structure and function of hexon proteins and adenovirus resulting from the HVRs.
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| http://dx.doi.org/10.1016/j.bbrc.2012.03.125 | DOI Listing |
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