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We have designed two highly polymorphic microsatellite multiplexes for Larix decidua Mill (European larch), a coniferous tree species with a fragmented distribution across Europe. The multiplexes combine microsatellites previously designed for the sister species L. kaempferi and newly identified microsatellites obtained by pyrosequencing of an enriched microsatellite library and subsequent marker candidate selection. As we wanted to target highly polymorphic markers, only microsatellite motifs with a high number of repeats (≥ 12) were selected. An important proportion of the marker candidates presented multiple bands, bad amplification or insufficient polymorphism. Such difficulties were expected owing to the large genome size of the studied species. Our strategy for marker validation followed most recent recommendations for microsatellite development, for example verifying marker quality in terms of polymorphism and accurate allele binning before multiplexing. The most promising loci were combined in two multiplexes, a 7-plex and a 6-plex. These were tested on a sample of 413 individuals from 18 populations distributed across the natural range. The 13 loci had from 9 to 36 alleles. Markers were successfully tested in another laboratory, confirming robustness of the marker protocols. We also tested transferability on six other larch species from Asia and North America. Overall, this study shows that, even in species with large genome size and relatively low overall polymorphism, microsatellites can be successfully developed using next-generation sequencing technologies, provided that some additional precautions are taken compared to species lacking these characteristics.
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http://dx.doi.org/10.1111/j.1755-0998.2012.03139.x | DOI Listing |
J Environ Manage
December 2024
Department of Ecology and Biogeography, Nicolaus Copernicus University in Toruń, Poland; Centre for Climate Change Research, Nicolaus Copernicus University in Toruń, Poland.
Tree Physiol
December 2024
Karlsruhe Institute of Technology, Institute of Meteorology and Climate Research - Atmospheric Environmental Research (KIT/IMK-IFU), Kreuzeckbahnstraße 19, 82467 Garmisch-Partenkirchen, Germany.
The severity of droughts is expected to increase with climate change, leading to more frequent tree mortality and a decline in forest ecosystem services. Consequently, there is an urgent need for monitoring networks to provide early warnings of drought impacts on forests. Dendrometers capturing stem diameter variations may offer a simple and relatively low-cost opportunity.
View Article and Find Full Text PDFSci Total Environ
December 2024
Research and Innovation Centre, Fondazione Edmund Mach, Via E. Mach 1, 38098 San Michele all'Adige, TN, Italy.
The use of correlation between tree-ring series as a proximity indicator in dendroprovenance analyses is often questioned. High correlations may occur between series at a great distance, but conversely, low correlations may occur between series that are close to each other. This discrepancy has prompted the exploration of alternative dendroprovenancing methods, but many of them have proven to be unreliable or impractical.
View Article and Find Full Text PDFClim Dyn
March 2024
Nature Rings - Environmental Research and Education, Mainz, Germany.
Environ Sci Technol
September 2024
Department of Forest Ecology and Management, Swedish University of Agricultural Sciences, Umeå SE-90183, Sweden.
Tree rings are an emerging atmospheric mercury (Hg) archive. Questions have arisen, though, regarding their mechanistic controls and reliability. Here, we report contrasting tree-ring Hg records in three collocated conifer species: Norway spruce (), Scots pine (), and European larch (), which are from a remote boreal forest.
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