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In vivo two-photon FLIM resolves photosynthetic properties of maize bundle sheath cells.
Photosynth Res
January 2025
State Key Laboratory of Forage Breeding-by-Design and Utilization, Key Laboratory of Photobiology, Institute of Botany, Chinese Academy of Sciences, Beijing, 100093, China.
Maize (Zea mays L.) performs highly efficient C photosynthesis by dividing photosynthetic metabolism between mesophyll and bundle sheath cells. In vivo physiological measurements are indispensable for C photosynthesis research as photosynthetic activities are easily interrupted by leaf section or cell isolation.
View Article and Find Full Text PDFVisualization of labeled micro- and nanoplastics in interaction with algae, using super-resolution stimulated emission depletion microscopy and fluorescence lifetime imaging.
Environ Toxicol Chem
January 2025
Blue Growth Research Lab, Ghent University, Ostend Science Park, Ostend, Belgium.
In contrast to microplastics, studying the interactions of nanoplastics (NPs) with primary producers such as marine microalgae remains challenging. This is attributed to the lack of adequate visualization methods that can distinguish NPs from autofluorescent biological material such as marine algae. The aim of this study was to develop a method for labeling and visualizing nonfluorescent micro- and nanoplastics (MNPs) of various polymer types, shapes, and sizes, in interaction with marine primary producers, which are autofluorescent.
View Article and Find Full Text PDFDevelopment of multifunctional fluorescence-emitting potential theranostic agents for Alzheimer's disease.
Talanta
January 2025
Pharmaceutical Chemistry Research Laboratory I, Department of Pharmaceutical Engineering & Technology, Indian Institute of Technology (Banaras Hindu University), Varanasi, 221005, India. Electronic address:
The cholinergic deficits and amyloid beta (Aβ) aggregation are the mainstream simultaneously observed pathologies during the progression of Alzheimer's disease (AD). Deposited Aβ plaques are considered to be the primary pathological hallmarks of AD and are contemplated as promising diagnostic biomarker. Herein, a series of novel theranostic agents were designed, synthesised and evaluated against cholinesterase (ChEs) enzymes and detection of Aβ species, which are major targets for development of therapeutics for AD.
View Article and Find Full Text PDFTowards measurements of absolute membrane potential in Bacillus subtilis using fluorescence lifetime.
Biophys Rep (N Y)
January 2025
UCLA-DOE Institute for Genomics and Proteomics, Department of Biological Chemistry, University of California at Los Angeles, Los Angeles, CA 90095, USA,; Department of Chemistry and Biochemistry, University of California at Los Angeles, Los Angeles, CA 90095, USA,; Department of Physiology, University of California at Los Angeles, Los Angeles, CA 90095, USA,; California Nano Systems Institute, University of California at Los Angeles, Los Angeles, CA 90095, USA,; Department of Physics, Institute for Nanotechnology and Advanced Materials, Bar-Ilan University, Ramat-Gan 52900, Israel.
Membrane potential (MP) changes can provide a simple readout of bacterial functional and metabolic state or stress levels. While several optical methods exist for measuring fast changes in MP in excitable cells, there is a dearth of such methods for absolute and precise measurements of steady-state membrane potentials (MPs) in bacterial cells. Conventional electrode-based methods for the measurement of MP are not suitable for calibrating optical methods in small bacterial cells.
View Article and Find Full Text PDFDefining the Zone of Acute Peripheral Nerve Injury Using Fluorescence Lifetime Imaging in a Crush Injury Sheep Model.
J Hand Surg Am
January 2025
Hand and Upper Extremity Division of Plastic and Reconstructive Surgery, University of California Davis, Sacramento, CA.
Purpose: Current technologies to define the zone of acute peripheral nerve injury intraoperatively are limited by surgical experience, time, cumbersome electrodiagnostic equipment, and interpreter reliability. In this pilot study, we evaluated a real-time, label-free optical technique for intraoperative nerve injury imaging. We hypothesize that fluorescence lifetime imaging (FLIm) will detect a difference between the time-resolved fluorescence signatures for acute crush injuries versus uninjured segments of peripheral nerves in sheep.
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