We developed a video recording system with the capability of tracking moving objects and used it to track the flight of an insect. The system consists of two galvano mirrors, which redirect the light coming from the object in two orthogonal directions toward a high-speed camera to capture the image. An additional high-speed camera, which views the same object through a beam splitter placed between one of the galvano mirrors and the observation camera, detects the position of the object. The mirror angle is controlled to maintain the position of the object at the center of the view, allowing the object to be tracked. In order to validate this system, images of a live fly in flight were recorded along a flight path that was much longer than the field of view of the stationary camera. A high-resolution video image of a rapidly moving live fly was successfully captured.
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http://dx.doi.org/10.1063/1.3694569 | DOI Listing |
Sensors (Basel)
June 2024
Namiki Laboratory, Graduate School of Engineering, Chiba University, 1-33 Yayoi-cho, Inage-ku, Chiba-shi 263-8522, Chiba, Japan.
Active vision systems (AVSs) have been widely used to obtain high-resolution images of objects of interest. However, tracking small objects in high-magnification scenes is challenging due to shallow depth of field (DoF) and narrow field of view (FoV). To address this, we introduce a novel high-speed AVS with a continuous autofocus (C-AF) approach based on dynamic-range focal sweep and a high-frame-rate (HFR) frame-by-frame tracking pipeline.
View Article and Find Full Text PDFSmall
December 2023
Center for Advanced Manufacturing, University of Southern California, Los Angeles, CA, 90007, USA.
Three-dimensional (3D) printing methods, such as vat photopolymerization (VPP) and direct-ink-writing (DIW) processes, are known for their high-resolution and multimaterial capabilities, respectively. Here a novel hybrid 3D printing technique that combines the strengths of VPP and DIW processes to achieve multimaterial and high-resolution printing of functional structures and devices, is presented. The method involves dispensing liquid-like materials via syringes into a photocurable matrix material and subsequently using a Galvano mirror-controlled laser beam to selectively photocure the dispensed material trace or the matrix material surrounding the trace.
View Article and Find Full Text PDFAnal Chem
September 2023
College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, People's Republic of China.
In recent years, optical tweezers have become a novel tool for biodetection, and to improve the inefficiency of a single trap, the development of multitraps is required. Herein, we constructed a set of hybrid multitrap optical tweezers with the balance of stability and flexibility by the combination of two different beam splitters, a diffraction optical element (DOE) and galvano mirrors (GMs), to capture polystyrene (PS) microbeads in aqueous solutions to create an 18-trap suspended array. A sandwich hybridization strategy of DNA-miRNA-DNA was adopted to detect three kinds of target miRNAs associated with triple negative breast cancer (TNBC), in which different upconversion nanoparticles (UCNPs) with red, green, and blue emissions were applied as luminescent tags to encode the carrier PS microbeads to further indicate the levels of the targets.
View Article and Find Full Text PDFJ Biomech Eng
February 2023
Human Performance Laboratory, University of Calgary, Calgary, AB T2N 1N4, Canada.
The deformation of articular cartilage and its cells at the micro-scale during dynamic activities such as gait has high mechanoregulatory importance. Measuring the cellular geometries during such dynamics has been limited by the rate of microscopic image acquisition. The introduction of resonating mirrors for image rasterization (resonant scanning), rather than the conventional servo control (galvano scanning), has significantly improved the scanning rate by more than 100×.
View Article and Find Full Text PDFMolecules
May 2022
Molecular Therapeutics Program, Fox Chase Cancer Center, Philadelphia, PA 19111, USA.
Many important biological processes such as protein folding and ligand binding are too fast to be fully resolved using conventional stopped-flow techniques. Although advances in mixer design and detection methods have provided access to the microsecond time regime, there is room for improvement in terms of temporal resolution and sensitivity. To address this need, we developed a continuous-flow mixing instrument with a dead time of 12 to 27 µs (depending on solution viscosity) and enhanced sensitivity, sufficient for monitoring tryptophan or tyrosine fluorescence changes at fluorophore concentrations as low as 1 µM.
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