Center for BioEnergetics, Biodesign Institute, Arizona State University, Tempe, Arizona 85287-6301, USA.
Published: April 2012
AI Article Synopsis
Article Abstract
Structure-activity studies were employed to investigate the stabilization of DNA-topoisomerases I and II covalent binary complexes by topopyrone analogues. The synthesis of five new topopyrone derivatives and study of their ability to stabilize DNA-topoisomerase I and DNA-topoisomerase II covalent binary complexes are described. The biochemical assays suggest that the orientation of the fused 1,4-pyrone ring and halogen substituents contribute importantly to the overall potency of the topopyrones as topoisomerase poisons.
The design and implementation of collective actions in model protocell communities is an on-going challenge in synthetic protobiology. Herein, we covalently graft alginate or chitosan onto the outer surface of semipermeable enzyme-containing silica colloidosomes to produce hairy catalytic protocells with pH-switchable membrane surface charge. Binary populations of the enzymatically active protocells exhibit self-initiated stimulus-responsive changes in spatial organization such that the mixed community undergoes alternative modes of electrostatically induced self-sorting and reversible co-clustering.
Poor aqueous solubility of crystalline active pharmaceutical ingredients (APIs) restricts their bioavailability. Amorphous solid dispersions with biocompatible polymer excipients offer a solution to overcome this problem, potentially enabling a broader use of many drug candidate molecules. This work addresses various aspects of the design of a suitable combination of an API and a polymer to form such a binary solid dispersion.
mRNA is crucial for various biomedical applications, but safe and efficient delivery into target cells is key for its effectiveness.
A new ternary delivery system was developed using linear poly(β-amino ester)s (LPAEs) coated with hyaluronic acid (HA), which significantly improves mRNA transfection efficiency and cell viability.
The HA coating enhances the properties of the polyplexes, increasing their ability to interact with cell membranes, suggesting this method could benefit other non-viral mRNA delivery systems.
Chalcogen bonds (ChBs) involving selenium have attracted substantial scholarly interest in past years owing to their fundamental roles in various chemical and biological fields. However, the effect of the valency state of the electron-deficient selenium atom on the characteristics of such ChBs remains unexplored. Herein, we comparatively studied the σ-hole-type Se∙∙∙O ChBs between SeF/SeF and a series of oxygen-bearing Lewis bases, including water, methanol, dimethyl ether, ethylene oxide, formaldehyde, acetaldehyde, acetone, and formic acid, using ab initio computations.
Zhejiang Provincial Key Lab for Biological and Chemical Processing Technologies of Farm Product, School of Biological and Chemical Engineering, Zhejiang University of Science and Technology, Hangzhou 310023, Zhejiang, China. Electronic address:
Curcumin (Cur) has many potential applications in the food industry. However, it suffers from poor water solubility, low plasma and tissue uptake and susceptibility to oxidation. In this study, β-lactoglobulin (β-LG)-hyaluronic acid (HA) binary covalent complexes were prepared by ultrasound-assisted Maillard reaction and used as the carrier to deliver Cur.
