Ann Agric Environ Med
Department of Bacteriology, National Institute of Public Health, Warsaw, Poland.
Published: July 2012
The aim of current studies was to validate the direct plating of a stool sample for Listeria monocytogenes detection, using selective medium Palcam agar with Palcam selective supplement. Validation was performed using stool samples collected from healthy humans inoculated with Listeria sp. strains. Stool samples were frozen to determine the influence of freezing on method robustness. The presented research defines the Listeria monocytogenes limit of detection (LOD) as 10(3) cfu/g of stools for fresh and frozen samples. Repeatability and reproducibility of the method has been confirmed using statistical methods. We show the effectiveness of direct plating of stool samples on Palcam agar with Palcam selective supplement collected for Listeria monocytogenes detection. This method could be useful for this pathogen detection in stool samples collected from patients with diarrhoea.
Download full-text PDF |
Source |
---|
Waste Manag
January 2025
Centre for Nutrition and Food Sciences, Queensland Alliance for Agriculture and Food Innovation (QAAFI), The University of Queensland, Brisbane, Queensland 4072, Australia; School of Agriculture and Food Sustainability, Faculty of Science, University of Queensland, Brisbane, Queensland 4072, Australia.
Black soldier fly larvae (BSFL) can valorise different organic matter and yield a product of high nutritional value. The lack of knowledge about the microbial safety of BSFL grown on different organic waste streams influences the commercialisation of BSFL as stockfeed ingredient. This study evaluates the microbial safety of BSFL grown on five different commercial food waste streams collected from two commercial production facilities.
View Article and Find Full Text PDFInt J Food Microbiol
January 2025
Department of Food Science and Biotechnology, Dongguk University, Goyang 10326, Republic of Korea. Electronic address:
Spent coffee grounds (SCGs) have been explored for use as various bioresources, such as biofuels, and are known to possess biological functions, including antioxidant activity. However, the antibiofilm properties of SCGs against pathogenic bacteria have not been fully investigated. Therefore, this study aimed to highlight the inhibitory effects of SCG extract (SCGE) on biofilm formation in Listeria monocytogenes and investigated the underlying mechanisms.
View Article and Find Full Text PDFAnal Chem
January 2025
Tianjin Key Laboratory of Food Science and Health, School of Medicine, Nankai University, Tianjin 300071, China.
() is one of the most prevalent threats, capable of inducing diverse illnesses and presenting a serious threat to public health. Herein, we demonstrate a novel dual-mode colorimetric/fluorescence biosensor based on the xponential mplification reaction and trand isplacement eactions (EASDR), which has multiplexing capability that significantly promotes the anchoring and trapping of Pt nanoparticles (Pt NPs) and fluorescent dyes for sensitive detection of (). The method works by targeting specific bacteria with aptamers and promoting repeated EASDR to affect the immobilization of Pt NPs and fluorescent dyes in the orifice plate, which could produce changes in fluorescence and colorimetric signals.
View Article and Find Full Text PDFCell Rep
January 2025
Interfaculty Institute of Microbiology and Infection Medicine, University of Tübingen, 72076 Tübingen, Baden-Württemberg, Germany; Cluster of Excellence EXC 2124 Controlling Microbes to Fight Infections, University of Tübingen, 72076 Tübingen, Baden-Württemberg, Germany. Electronic address:
Epithelial cells respond to infection with the intracellular bacterial pathogen Listeria monocytogenes by altering their mechanics to promote collective infected cell extrusion (CICE) and limit infection spread across cell monolayers. However, the underlying biochemical pathways remain elusive. Here, using in vitro (epithelial monolayers) and in vivo (zebrafish larvae) models of infection with L.
View Article and Find Full Text PDFHeliyon
January 2025
Lithuanian University of Health Sciences, Faculty of Medicine, A.Mickeviciaus street, 9, LT-44307, Kaunas, Lithuania.
Introduction: is a formidable pathogen that poses a significant threat to immunocompromised and might cause rare atypical forms of the disease especially complicated with coinfection.
Case: We present a case of a patient with meningoencephalitis, endocarditis, sepsis, and osteomyelitis, highlighting the complexities of managing disseminated polymicrobial infection. A 64-year-old female with multiple myeloma treated with chemotherapy presented with fever, altered mental status, nausea, and diarrhea to the emergency department.
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!
© LitMetric 2025. All rights reserved.