AI Article Synopsis
- The (R)-specific enoyl-CoA hydratase gene (phaJ(HS21)) from Pseudomonas chlororaphis HS21 was overexpressed in different Pseudomonas strains to create polyhydroxyalkanoates (PHAs) with new monomer compositions.
- Recombinant strains containing both phaC(HS21) and phaJ(HS21) produced PHAs with saturated and unsaturated monomers ranging from C12 to C14.
- The level of the monomer 3-hydroxytetradecenoate significantly increased by about 260% in the recombinant P. chlororaphis HS21, suggesting that PhaJ(HS21) could be valuable for creating
Article Abstract
The (R)-specific enoyl-CoA hydratase gene (phaJ(HS21)) from Pseudomonas chlororaphis HS21 was overexpressed in various Pseudomonas strains, alone and in combination with the polyhydroxyalkanoate synthase gene (phaC(HS21)), for the biosynthesis of polyhydroxyalkanoates (PHAs) of altered monomer composition. Recombinant Pseudomonas strains harboring phaC(HS21) and phaJ(HS21) generated saturated and unsaturated monomers of C12-C14 in their PHAs. In particular, the level of the 3-hydroxytetradecenoate monomer in recombinant P. chlororaphis HS21 increased by approximately 260%. PhaJ(HS21) is expected to be useful in the biosynthesis of PHAs consisting of unusual monomer units.
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| http://dx.doi.org/10.1271/bbb.110871 | DOI Listing |
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