Animals were immunized with transmissible gastroenteritis virus conjugated with gold nanoparticles. The resultant antibodies had a higher titer than antibodies produced in response to native virus. Immunization with the antigen-colloidal gold complex led to a significant increase of the peritoneal macrophages respiratory activity and of plasma IFN-γ level in immunized animals.
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http://dx.doi.org/10.1007/s10517-011-1350-8 | DOI Listing |
Anal Chem
January 2025
Key Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education, Fujian Provincial Key Laboratory of Photonics Technology, Fujian Normal University, Fuzhou, Fujian 350117, China.
Multiple myeloma is a hematologic malignancy characterized by the proliferation of abnormal plasma cells in the bone marrow. Despite therapeutic advancements, there remains a critical need for reliable, noninvasive methods to monitor multiple myeloma. Circulating plasma cells (CPCs) in peripheral blood are robust and independent prognostic markers, but their detection is challenging due to their low abundance.
View Article and Find Full Text PDFNanomaterials (Basel)
January 2025
European Commission, Joint Research Centre (JRC), 21027 Ispra, Italy.
The evaluation of nanoplastics bioaccumulation in living organisms is still considered an emerging challenge, especially as global plastic production continues to grow, posing a significant threat to humans, animals, and the environment. The goal of this work is to advance the development of standardized methods for reliable biomonitoring in the future. It is crucial to employ sensitive techniques that can detect and measure nanoplastics effectively, while ensuring minimal impact on the environment.
View Article and Find Full Text PDFNanomaterials (Basel)
January 2025
Materials Measurement Laboratory, National Institute of Standards and Technology, Gaithersburg, MD 20899, USA.
The safety screening of manufactured nanomaterials (MNMs) is essential for their adoption by consumers and the marketplace. Lately, animal-based testing has been replaced by mechanistically informative in vitro assays due to the requirements of regulatory agencies. Cell viability assays are widely employed for manufactured nanomaterial hazard screening as a first-tier approach.
View Article and Find Full Text PDFBiosensors (Basel)
January 2025
School of Chemistry and Chemical Engineering, Linyi University, Linyi 276005, China.
MicroRNA122 (miR-122) is a microRNA that is highly expressed in hepatocytes and has been identified as a prospective therapeutic target and biomarker for liver injury. An expanding body of research has demonstrated that miR-122 is a critical regulator in both the initiation and progression of a wide range of liver diseases. Traditional methods for detecting miR-122 mainly include Northern blotting and qRT-PCR, but they are technically complex and cumbersome, requiring expensive instruments and high technical requirements.
View Article and Find Full Text PDFBiosensors (Basel)
January 2025
Furong Labratory, Changsha 410083, China.
A fluorescence probe for "switch-on" detection of alkaline phosphatase (ALP) was developed based on Au nanoclusters anchored MnO nanosheets (Au NCs-MnO NSs), which were synthesized using bovine serum albumin (BSA) as template through a simple one-pot approach. In the sensing system, MnO NSs function as both energy acceptors and target identifiers, effectively quenches the fluorescence of Au NCs via fluorescence resonance energy transfer (FRET). The presence of ALP catalyzes the hydrolysis of L-ascorbic acid-2-phosphate (AAP) to ascorbic acid (AA), reducing MnO NSs to Mn and facilitate the fluorescence recovery of Au NCs.
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