The swimming behaviors of Tetrahymena can be used in sensitive behavioral bioassays for estimating the effects of drugs, mutations, and other conditions on the physiological state of the cell. These assays can be used in both forward and reverse genetic approaches to help understand cellular functions from genotype to phenotype.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/B978-0-12-385967-9.00015-3 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
SPOP enhances FADD degradation and decreases the activeness of the NF-κB signaling pathway in prostate cancer: an study.
Transl Androl Urol
December 2024
Department of Urology, Xinjiang Medical University Affiliated Tumor Hospital, Urumqi, China.
Background: Speckle-type POZ protein (SPOP), FAS-associated protein with death domain (FADD), and nuclear transcription factor-κB (NF-κB) have been shown to be associated with the development of prostate cancer (PCa). FADD has been shown to activate the NF-κB pathway to promote tumorigenesis, while SPOP has been shown to enhance the breakdown of FADD and inhibit the function of the NF-κB signaling pathway in non-small cell lung cancer. The existence of this mechanism has not yet been confirmed in PCa.
View Article and Find Full Text PDFClinical significance and pro-oncogenic function of DBF4 in clear cell renal cell carcinoma.
BMC Urol
January 2025
Institute of Clinical Medicine, The Second affiliated Hospital of Hainan Medical University, 368th Yehai Avenue, Haikou, Hainan, 570311, China.
Background: Clear cell renal cell carcinoma (ccRCC) is the most common malignant urological tumor, and regrettably, and is insensitive to chemotherapy and radiotherapy, resulting in poor patient outcomes. DBF4 plays a critical role in DNA replication and participates in various biological functions, making it an attractive target for cancer treatment. However, its significance in ccRCC has not yet been explored.
View Article and Find Full Text PDFUnraveling the in vivo pharmacokinetic behavior of mPEG-NH polymer in rats by UHPLC-MS/MS assay.
J Chromatogr B Analyt Technol Biomed Life Sci
January 2025
School of Chemical Engineering, Ocean and Life Sciences, Dalian University of Technology, Panjin, Liaoning, 124221, China. Electronic address:
As an important chemical reagent, methoxy polyethylene glycol amine (mPEG-NH) is widely used in biomedical field. Unraveling the pharmacokinetic behavior of mPEG-NH polymers is essential for revealing the toxicity and efficiency of mPEG-NH related drug delivery systems. In this study, a simple analytical assay based on mass spectrometry (MS) was first established and validated for quantification of mPEG-NH in biological matrix.
View Article and Find Full Text PDFVolatile cues of enhanced attractiveness to Parapanteles hyposidrae (Wilkinson) wasps mediated by jasmonic and salicylic acid pathways synergism in tea plant.
Pest Manag Sci
January 2025
Tea Research Institute, Chinese Academy of Agricultural Sciences, Ministry of Agriculture and Rural Affairs Key Laboratory of Tea Biology and Resource Utilization, Hangzhou, China.
Background: The jasmonic acid (JA) and salicylic acid (SA) pathways are often thought to interact antagonistically in plants when mediating anti-herbivore resistance. However, we previously found that the two pathways in tea plant interact synergistically when treated with 1.5 mmol/L methyl jasmonate (MeJA) and 20 mmol/L SA at 12 h intervals (MeJA+SA treatment).
View Article and Find Full Text PDFIn Vitro Assay to Examine Osteoclast Resorptive Activity Under Estrogen Withdrawal.
Bio Protoc
January 2025
Department of Biological Sciences, University of Toronto Scarborough, Toronto, ON, Canada.
The bone is a highly dynamic organ that undergoes continuous remodeling through an intricate balance of bone formation and degradation. Hyperactivation of the bone-degrading cells, the osteoclasts (OCs), occurs in disease conditions and hormonal changes in females, resulting in osteoporosis, a disease characterized by altered microarchitecture of the bone tissue, and increased bone fragility. Thus, building robust assays to quantify OC resorptive activity to examine the molecular mechanisms underlying bone degradation is critical.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!