AI Article Synopsis
- A 21-kDa leptospiral lipoprotein (LipL21) was tested for diagnosing bovine leptospirosis using ELISA, comparing both native and recombinant forms.
- The study found no significant difference in diagnostic efficiency between native LipL21 (nLipL21) and recombinant LipL21 (rLipL21).
- The rLipL21 ELISA showed 100% sensitivity with positive samples and 97.09% specificity with negative samples, suggesting it could serve as a reliable alternative to the traditional microscopic agglutination test (MAT).
Article Abstract
A 21-kDa leptospiral lipoprotein (LipL21) was evaluated for its diagnostic potential to detect bovine leptospirosis by ELISA. Both native LipL21 (nLipL21) and recombinant LipL21 (rLipL21) proteins were tested and compared regarding diagnostic efficiency, and no statistically significant difference was observed. The sensitivity of rLipL21 ELISA for 62 microscopic agglutination test (MAT) positive sera was 100% and the specificity with 378 MAT negative sera was 97.09%. Thus, rLipL21 protein-based ELISA could be used as an alternative to MAT for the diagnosis of bovine leptospirosis.
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Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3317464 | PMC |
| http://dx.doi.org/10.4142/jvs.2012.13.1.99 | DOI Listing |
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