AI Article Synopsis
- Developed a PCR-based kit called IDtyper X-16 for typing 16 specific X-STR loci on the X chromosome, utilizing a multiplex PCR system with fluorescent labeling.
- The study analyzed the polymorphisms among the loci, finding that 14 markers were highly polymorphic and two were moderately polymorphic, which enhances the kit's effectiveness.
- The IDtyper X-16 demonstrated exceptional discrimination power for identifying individuals and is particularly useful for forensic applications and paternity testing.
Article Abstract
Objective: To develop a PCR-based X-STR kit for typing of 16 X-STR loci and investigate the polymorphisms of the X-STR markers.
Methods: Sixteen STR loci (GATA 165B12, DXS101, GATA 172D05, HPRTB, DXS981, DXS8378, DXS6795, GATA 31E08, DXS6809, DXS6803, DXS9902, DXS6807, DXS7423, DXS7133, DXS6810 and DXS7132) located on X chromosome were selected. The primers for multiplex PCR were designed by Primer Premier 5.0 software and labeled by four fluorescences (FAM, HEX, TAMRA and ROX). The developed multiplex PCR system was used for investigating the polymorphisms of the X-STR markers in Han populations.
Results: The 16-plex amplification system named IDtyper X-16 was successfully developed and validated. Among the 16 X-STR loci, DXS7133 and DXS7423 were found to be moderately polymorphic and the other 14 X-STR markers were highly polymorphic (P1C > 0.5, H > 0.5). The cumulative discrimination power in females and in males were 0.999 999 999 999 97 and 0.999 999 993 respectively in Han population. The combined power of exclusion in trios and in duos were 0.999 999 93 and 0.999990, respectively.
Conclusion: The IDtyper X-16 kit is highly valuable in forensic science and is suitable for paternity testing in disputed cases.
Download full-text PDF |
Source |
|---|
Publication Analysis
Top Keywords
Similar Publications
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!