AI Article Synopsis
- The report details the creation and testing of a new RNA sample prep system, TruTip, for nasopharyngeal samples using a unique pipette tip design with a nucleic acid binding matrix.
- TruTip demonstrated extraction efficiencies comparable to the automated QIAcube method, successfully detecting influenza A and B at specific gene copy levels.
- Preclinical tests showed a 98% detection accuracy for TruTip compared to the established easyMAG extraction method, highlighting its potential for automation and adaptability.
Article Abstract
This report describes the development and pre-clinical testing of a new, random-access RNA sample preparation system (TruTip) for nasopharyngeal samples. The system is based on a monolithic, porous nucleic acid binding matrix embedded within an aerosol-resistant pipette tip and can be operated with single or multi-channel pipettors. Equivalent extraction efficiencies were obtained between automated QIAcube and manual TruTip methods at 10(6) gene copies influenza A per mL nasopharyngeal aspirate. Influenza A and B amended into nasopharyngeal swabs (in viral transport medium) were detected by real-time RT-PCR at approximately 745 and 370 gene copies per extraction, respectively. RNA extraction efficiency in nasopharyngeal swabs was also comparable to that obtained on an automated QIAcube instrument over a range of input concentrations; the correlation between threshold cycles (or nucleic acid recovery) for TruTip and QIAcube-purified RNA was R(2)>0.99. Preclinical testing of TruTip on blinded nasopharyngeal swab samples resulted in 98% detection accuracy relative to a clinically validated easyMAG extraction method. The physical properties of the TruTip binding matrix and ability to customize its shape and dimensions likewise make it amenable to automation and/or fluidic integration.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3348996 | PMC |
| http://dx.doi.org/10.1016/j.jviromet.2012.03.002 | DOI Listing |
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