AI Article Synopsis
- - The study investigated how nicotine affects the differentiation and functionality of dendritic cells (DCs), specifically human monocyte-derived DCs (NiDCs), finding that nicotine exposure led to decreased CD1a levels and suppressed secretion of key pro-inflammatory cytokines (IL-12 and TNF-α) in response to stimulation.
- - NiDCs exhibited a weaker ability to promote the proliferation of T cells and produce IFN-γ, along with enhanced expression of molecules that inhibit immune responses (coinhibitory molecules), which suggests impaired immune activation.
- - The research revealed that nicotine exposure increased levels of the nuclear receptor PPAR γ, which affects immune regulation, and that blocking a specific type of receptor (nACh
Article Abstract
We examined the effects of nicotine on differentiation and function of monocyte-derived human dendritic cells (DCs). NiDCs, which were the DCs differentiated in the presence of nicotine, showed lower levels of CD1a. Secretion of IL-12 and TNF-α by lipopolysaccharide (LPS)-stimulated NiDCs was significantly suppressed compared to monocyte-derived DCs grown without nicotine. NiDCs displayed a diminished capacity to induce allogeneic T cell proliferation with a reduced production of IFN-γ, and maintained/enhanced LPS-mediated expression of coinhibitory molecules. Interestingly, NiDCs enhanced the expression of nuclear receptor peroxisome proliferator-activated receptors γ (PPAR γ), which has immunomodulatory properties. Expression of PPAR γ and PPAR γ-target genes was significantly inhibited by pretreatment with d-tubocurarine, antagonist of non-selective nicotinic acetylcholine receptors (nAChR). In addition, reduction of Th1 responses was inhibited after blocking nAChR-mediated signal. These data suggest the effect of nicotine on altering DC immunogenicity by impeding Th1 immunity is partially mediated by upregulation of PPAR γ.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.cellimm.2012.02.007 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!