Previous metagenomic analysis indicated that numerous insect viruses exist in bat guano. In this study, we isolated a novel double-stranded RNA virus, a tentative member of the family Totiviridae, designated Tianjin totivirus (ToV-TJ), from bat feces. The virus is an icosahedral particle with a diameter of 40-43 nm, and it causes cytopathic effect in Sf9, Hz, and C6/36 cell lines. Full-length genomic sequence analysis showed that ToV-TJ shares high similarity with the totivirus OMRV-AK4, which was recently isolated from mosquitoes in Japan. The full-length genome of the ToV-TJ was 7611 bp and contained two predicted non-overlapping open reading frames (ORFs): ORF1, encoding the capsid protein (CP), and ORF2, encoding an RNA-dependent RNA polymerase. Bioassay of ToV-TJ by feeding on the larvae of Spodoptera exigua and Helicoverpa armigera (Hubner) suggests that this virus is not infectious for these two larvae in vivo. Sequences similar to that of ToV-TJ have been detected in bat feces sampled in Yunnan and Hainan Provinces, suggesting that this virus is widely distributed.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1007/s00705-012-1278-y | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Habitat sharing and interspecies interactions in caves used by bats in the Republic of Congo.
PeerJ
January 2025
CIRAD, UMR ASTRE, Montpellier, France.
Bats play key roles in ecosystem functions and provide services to human populations. There is a need to protect bat populations and to mitigate the risks associated with pathogen spillover. Caves are key habitats for many bat species, which use them as roosting and breeding sites.
View Article and Find Full Text PDFPredators in the Dark: Metabarcoding Reveals Arcellinida Communities Associated with Bat Guano, Endemic to Dinaric Karst in Croatia.
Microb Ecol
January 2025
Real Jardín Botánico (RJB-CSIC), C/ Moyano 1, 28014, Madrid, Spain.
Article Synopsis
- Karst caves are unique ecosystems with low organic matter and high endemic biodiversity, particularly in microbes like testate amoebae (Arcellinida).
- Recent research in Dinaric karstic caves, using metabarcoding techniques, reveals that bat guano significantly enhances the diversity and abundance of Arcellinida communities.
- Findings suggest that bat guano creates rich habitats for unique Arcellinida species, emphasizing the important ecological role of bats in these subterranean environments.
Disseminated Histoplasmosis With Lung and Liver Involvement in an Immunocompetent Patient.
Cureus
December 2024
Nephrology, Hospital Clinic Barcelona, Barcelona, ESP.
Histoplasmosis, caused by the fungus , is a significant public health concern in endemic regions like Mexico. Immunocompromised individuals, especially those with HIV infection and those exposed to nitrogen-rich environments, such as bird excrement or bat guano, are particularly vulnerable. This case report describes a middle-aged patient with jaundice in the skin and mucous membranes.
View Article and Find Full Text PDFHistoplasmosis Associated With Bat Guano Exposure in Cannabis Growers: 2 Cases.
Open Forum Infect Dis
December 2024
Associate Professor of Medicine, Department of Infectious Disease at the University of Rochester, Rochester, New York, USA.
is a pathogenic dimorphic fungus with evolving epidemiology. Initially described as endemic to the Ohio and Mississippi river valleys, the infection now regularly occurs in central and eastern United States, with cases reported across the entire country. Transmission happens via inhalation of conidia during activities that disturb fungal hyphae.
View Article and Find Full Text PDFThe spatiotemporal and genetic architecture of extraoral taste buds in Astyanax cavefish.
Commun Biol
August 2024
Department of Biological Sciences, University of Cincinnati, Cincinnati, OH, USA.
Intense environmental pressures can yield both regressive and constructive traits through complex evolutionary mechanisms. Although regression is well-studied, the biological bases of constructive features are less well understood. Cave-dwelling Astyanax fish harbor prolific extraoral taste buds on their heads, which are absent in conspecific surface-dwellers.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!