AI Article Synopsis

  • The exchange of macromolecules between the nucleus and cytoplasm is crucial for regulating gene expression in cells, relying on nuclear pore complexes (NPCs) made of proteins called nucleoporins (Nups) and transport factors for cargo movement.
  • The NPC not only facilitates transport but also influences nuclear structure and gene expression control.
  • Research using the yeast Saccharomyces has provided insights into the NPC's structure, function, and its interactions with transport factors and regulatory elements, highlighting the system's fundamental and conserved features.

Article Abstract

Exchange of macromolecules between the nucleus and cytoplasm is a key regulatory event in the expression of a cell's genome. This exchange requires a dedicated transport system: (1) nuclear pore complexes (NPCs), embedded in the nuclear envelope and composed of proteins termed nucleoporins (or "Nups"), and (2) nuclear transport factors that recognize the cargoes to be transported and ferry them across the NPCs. This transport is regulated at multiple levels, and the NPC itself also plays a key regulatory role in gene expression by influencing nuclear architecture and acting as a point of control for various nuclear processes. Here we summarize how the yeast Saccharomyces has been used extensively as a model system to understand the fundamental and highly conserved features of this transport system, revealing the structure and function of the NPC; the NPC's role in the regulation of gene expression; and the interactions of transport factors with their cargoes, regulatory factors, and specific nucleoporins.

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3296253PMC
http://dx.doi.org/10.1534/genetics.111.127803DOI Listing

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