AI Article Synopsis
- * Researchers evaluated the IgA-uromodulin complex in urine samples from patients to establish its potential as a diagnostic marker using various analytical methods like LC-MS and ELISA.
- * Results showed that the IgA-uromodulin complex was highly prevalent in IgAN patients (81.7% positive), indicating that its detection through ELISA could effectively diagnose IgAN non-invasively.
Article Abstract
Background: The only tool to diagnose immunoglobulinn A nephropathy (IgAN) is renal biopsy which requires hospitalization; moreover, renal biopsy has a risk of critical bleeding. Therefore, a non-invasive method for accurate diagnosis of IgAN is desirable and a must-to-have tool for the clinics. For this purpose, we evaluated the diagnostic value of the IgA-uromodulin complex in the urine of patients with IgAN for its feasibility and adequacy.
Method: We determined the IgA-uromodulin complex as a candidate for a diagnostic marker of IgAN by immunoprecipitation, liquid chromatography-mass spectrometry (LC-MS) and Western blot analysis. The enzyme-linked immunosorbent assay (ELISA) for the IgA-uromodulin complex was developed and applied to urine samples obtained from various kidney disease patients.
Result: One hundred and three of 126 urine samples (81.7%) from IgAN patients were positive for the IgA-uromodulin complex, while only 25 out of 94 urine samples (26.6%) in other kidney disease patients were positive. Sensitivity was 81.7%, specificity was 73.4%, and diagnosis efficiency was 78.2%. The complex was negative in eight urine samples obtained from patients with Alport syndrome which is almost impossible to discriminate from IgAN by routine urinalysis.
Conclusion: Detection of the urinary IgA-uromodulin complex by ELISA is a useful non-invasive method to diagnose IgAN.
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|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3465549 | PMC |
| http://dx.doi.org/10.1007/s10157-012-0617-3 | DOI Listing |
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Article Synopsis
- * Researchers evaluated the IgA-uromodulin complex in urine samples from patients to establish its potential as a diagnostic marker using various analytical methods like LC-MS and ELISA.
- * Results showed that the IgA-uromodulin complex was highly prevalent in IgAN patients (81.7% positive), indicating that its detection through ELISA could effectively diagnose IgAN non-invasively.
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