Laboratoire des Polymères, Institut des Matériaux et Institut des Sciences et Ingénieries Chimiques, Ecole Polytechnique Fédérale de Lausanne, CH-1015 Lausanne, Switzerland.
Published: May 2012
AI Article Synopsis
Article Abstract
Three synthesis lots of linear poly(ethyleneimine) (PEI) are compared to a fully hydrolyzed linear PEI (commercially available as PEI "Max") regarding structure, polyplex formation with plasmid DNA, and transfection of suspension-adapted HEK-293E cells. PEI "Max" binds DNA more efficiently than the other PEIs, but it is the least effective in terms of transient recombinant protein yield. One PEI lot is fractionated by means of SEC. The fractions of high-M(n) PEI are the most efficient for complex formation and transfection. Nevertheless, the highest transient recombinant protein yields are achieved with unfractionated PEI. The results demonstrate that the polydispersity and charge density of linear PEI are important parameters for gene delivery to suspension-adapted HEK-293E cells.
Imbalances in gut microbiota and their metabolites have been implicated in osteoporotic disorders. Trimethylamine-n-oxide (TMAO), a metabolite of L-carnitine produced by gut microorganisms and flavin-containing monooxygenase-3, is known to accelerate tissue metabolism and remodeling; however, its role in bone loss remained unexplored. This study investigates the relationship between gut microbiota dysbiosis, TMAO production, and osteoporosis development.
Lower-limb exoskeletons have become increasingly popular in rehabilitation to help patients with disabilities regain mobility and independence. Brain-computer interface (BCI) offers a natural control method for these exoskeletons, allowing users to operate them through their electroencephalogram (EEG) signals. However, the limited EEG decoding performance of the BCI system restricts its application for lower limb exoskeletons.
Department of Biochemical Engineering, School of Chemical Engineering and Technology and Key Laboratory of Systems Bioengineering and Frontiers Science Center for Synthetic Biology (Ministry of Education), Tianjin University, Tianjin 300350, PR China. Electronic address:
Our previous studies on protein adsorption onto anion-exchangers of poly(ethylenimine) (PEI)-grafted Sepharose FF (PEI-Sepharose) proved their significantly improved performance over the commercial nongrafting anion-exchangers such as Q Sepharose FF, and it was found the protein adsorption behavior on PEI-Sepharose was more sensitive to counterions (Cl, SCN, HPO and SO). However, the complicated role of counterions has not been well interpreted due to their distinct chemical and physical characteristics. Thus, we have further studied the counterion effects by adding two halide ions (F and Br) to explore the effects of the three halide ions on bovine serum albumin adsorption and the results were compared with previous data.
Key Laboratory of Photonic and Electronic Bandgap Materials Ministry of Education, School of Physics and Electronic Engineering, Harbin Normal University, Harbin, 150025, China.
Extracting uranium from seawater is crucial for tapping oceanic resources vital to future energy supply. This study synthesized a novel nitrogen vacancy carbon nitride (NCN) grafted polyethyleneimine (PEI) composite material (NCNP). Experiments and molecular dynamics simulations reveal that NCNP effectively hinders the diffusion of uranyl ions (UO ) to the NCN surface, thereby inhibiting electron transfer reactions.
In bone tissue engineering, manufacturing bone tissue constructs that closely replicate physiological features for regenerative repair remains a significant challenge. In recent years, the advent of indirect 3D printing technology has overcome the stringent material demands, confined resolution, and structural control challenges inherent to direct 3D printing. By utilizing sacrificial templates, the natural structures and physiological functions of bone tissues can be precisely duplicated.
