Poly(glutamic acid) has been studied with a nanosecond T-jump experiment. A new experimental set-up based on the frequency-quadrupling of an 82 MHz Titanium-Sapphire laser allows rapid CD measurements to be performed. Combining time-resolved absorption and circular dichroism at 204 and 220 nm, we are able to measure precisely the unfolding relaxation time as well as the helical fraction evolution. We show that only CD at 220 nm is relevant to observe the unfolding of an alpha helix whereas no change is observed for CD at 204 nm. Conversely, both absorptions yield information on the dynamics of the process.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3292019 | PMC |
| http://dx.doi.org/10.3390/ijms13022239 | DOI Listing |
Publication Analysis
Top Keywords
nanosecond t-jump
8
t-jump experiment
8
polyglutamic acid
8
circular dichroism
8
experiment polyglutamic
4
acid circular
4
dichroism study
4
study polyglutamic
4
acid studied
4
studied nanosecond
4
Similar Publications
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!