AI Article Synopsis
- The nematode Caenorhabditis elegans is a key model organism in research due to its well-understood biology, particularly its N-glycan structures.
- Recent analyses showed that these N-glycans vary significantly at different developmental stages and between wild-type and mutant embryos.
- Findings indicated that wild-type embryos from liquid cultures had a less diverse oligosaccharide profile than those from agar plates, while mutant embryos exhibited distinct differences that suggest changes in cellular identity and glycan processing.
Article Abstract
The free-living nematode Caenorhabditis elegans is a well-characterized eukaryotic model organism. Recent glycomic analyses of the glycosylation potential of this worm revealed an extremely high structural variability of its N-glycans. Moreover, the glycan patterns of each developmental stage appeared to be unique. In this study we have determined the N-glycan profiles of wild-type embryos in comparison to mutant embryos arresting embryogenesis early before differentiation and causing extensive transformations of cell identities, which allows to follow the diversification of N-glycans during development using mass spectrometry. As a striking feature, wild-type embryos obtained from liquid culture expressed a less heterogeneous oligosaccharide pattern than embryos recovered from agar plates. N-glycan profiles of mutant embryos displayed, in part, distinct differences in comparison to wild-type embryos suggesting alterations in oligosaccharide trimming and processing, which may be linked to specific cell fate alterations in the embryos.
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| http://dx.doi.org/10.1007/s10719-012-9371-8 | DOI Listing |
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