The TaqMan real-time PCR assay was developed from the Blastomyces dermatitidis BAD1 gene promoter. The assay identified all haplotypes of B. dermatitidis and five of six positive paraffin-embedded tissues. The assay sensitivity threshold was 1 pg genomic DNA of the mold form and 2 CFU of the yeast form of B. dermatitidis. No cross-reactivity was observed against other fungal DNA. The assay allowed rapid (5-h) identification of B. dermatitidis from culture and from clinical specimens.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3347106 | PMC |
| http://dx.doi.org/10.1128/JCM.00310-12 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Biomarker microRNA-371a-3p - expression in malignancies other than germ-cell tumours.
J Cancer Res Clin Oncol
January 2025
Department of Urology, Asklepios Tumorzentrum Hamburg, AK Altona, Hamburg, Germany.
Purpose: microRNA-371a-3p (M371) is considered a highly sensitive and specific serum biomarker of testicular germ cell tumours (GCTs). However, little is known about the expression of M371 in nontesticular malignancies (NTMs), so far. As knowledge about the expression of the marker in other malignancies is a prerequisite for the clinical application of the test we aimed to explore the M371 expression in other cancers.
View Article and Find Full Text PDFEnhanced vascular contraction induced by exposure to angiotensin II mediated by endothelin-1 biosynthesis following PKCβ activation.
Am J Physiol Heart Circ Physiol
January 2025
Laboratory of Veterinary Pharmacology, Faculty of Veterinary Medicine, Okayama University of Science, Imabari, Ehime, Japan.
Protein kinase C (PKC) reportedly plays a role in the pathogenesis of many vascular dysfunction-related conditions. In this study, we investigated whether PKCβ is associated with vascular contractile changes induced by angiotensin II (Ang II) exposure. Long-term (24 h) treatment of rat aortae and mesenteric arteries in Ang II-containing culture medium enhanced 5-hydroxytrypatamine (5-HT)-induced vascular contraction in a dose-dependent manner, in association with enhanced phosphorylation of PKCβ S660.
View Article and Find Full Text PDFMolecular diagnosis of infective endocarditis from culture-negative valve samples in a tertiary hospital in Iran.
Microbiol Spectr
January 2025
National Reference Laboratory for Plague, Tularemia and Q fever, Research Centre for Emerging and Reemerging Infectious Diseases, Pasteur Institute of Iran, Akanlu, Kabudar Ahang, Hamadan, Iran.
The aim of this study was to investigate the prevalence of and in valve samples from endocarditis patients using the real-time PCR method at a major referral heart hospital in Iran. In this study, 146 paraffin-embedded tissue samples from the heart valves of patients with clinical and pathological evidence of infective endocarditis (IE), who underwent heart valve replacement surgery between 2016 and 2020 at Tehran Heart Center were collected. After DNA extraction from paraffin-embedded valve tissue samples, they were surveyed for the presence of and using quantitative real-time PCR (qPCR).
View Article and Find Full Text PDF[Effect of separate and combined intake of rutin and hesperidin on the activity and gene expression of cytochrome P450 isoforms 1A and 3A in rat liver].
Vopr Pitan
January 2025
Federal Research Centre of Nutrition, Biotechnology and Food Safety, 109240, Moscow, Russian Federation.
The main molecular mechanisms of the protective effect of minor bioactive compounds (BAC) of food, including rutin and hesperidin, along with antiradical and antioxidant activity, include their interaction with transcription factors modulating the functional state of the organism defense systems, one of which is the system of xenobiotic metabolizing enzymes. However, the data on their combined action are limited. of the research was to study the effect of rutin and hesperidin on the activity and gene expression of cytochrome P450 isoforms 1A and 3A (CYP1A1, CYP1A2, CYP3A) in rat liver at their separate and combined intake.
View Article and Find Full Text PDFA study to determine the effect of nano-selenium and thymoquinone on the Nrf2 gene expression in Alzheimer's disease.
Future Sci OA
December 2025
Department of Biochemistry, Faculty of Dentistry, Future University, Cairo, Egypt.
Introduction: Alzheimer's disease is a developing public health concern in aging communities that affects a sizable section of the global population. The risk of Alzheimer's disease increases with age; it affects one-third of males and two-thirds of women This research attempts to assess the effect of nano-selenium and thymoquinone on Nrf2 gene expression levels in Alzheimer's disease (AD).
Methods: There were five identical groups of 50 albino male rats: a control group that was healthy; an AD positive control group; an AD group that received nano-selenium (5 mg/kg); an AD group that received thymoquinone (2 mg/kg); and an AD group that received both.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!