Objective: To establish a rapid STR genotyping method for individual identification.
Methods: Two hundred blood samples from FTA were collected. Equal amount of blood were collected by puncher and analyzed using two methods (6+1 STR kit in combination with EX-Q20 electrophoresis and Sinofiler kit in combination with POP4 electrophoresis). Consuming time and results of two methods were compared.
Results: 6+1 STR kit in combination with EX-Q20 electrophoresis method can obtain all genotyping results and be shorter time.
Conclusion: 6+1 STR kit in combination with EX-Q20 electrophoresis method is used to STR genotyping with accurate, reliable results and this new method is potential value in mass personnel investigation and comparison in major criminal cases. It also can raise the work efficiency.
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Discov Nano
January 2025
Institute of Physiology II, University of Münster, Robert-Koch-Str. 27b, 48149, Münster, Germany.
Metastatic cancer cells undergo metabolic reprogramming, which involves changes in the metabolic fluxes, including endocytosis, nucleocytoplasmic transport, and mitochondrial metabolism, to satisfy their massive demands for energy, cell division, and proliferation compared to normal cells. We have previously demonstrated the ability of two different types of compounds to interfere with linchpins of metabolic reprogramming, Pitstop-2 and 1,6-hexanediol (1,6-HD). 1,6-HD disrupts glycolysis enzymes and mitochondrial function, enhancing reactive oxygen species production and reducing cellular ATP levels, while Pitstop-2 impedes clathrin-mediated endocytosis and small GTPases activity.
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December 2024
Institute of Materials Science, Technische Universität Darmstadt, Peter-Grünberg-Str. 2, D-64287, Darmstadt, Germany.
The design of cathode/electrolyte interfaces in high-energy density Li-ion batteries is critical to protect the surface against undesirable oxygen release from the cathodes when batteries are charged to high voltage. However, the involvement of the engineered interface in the cationic and anionic redox reactions associated with (de-)lithiation is often ignored, mostly due to the difficulty to separate these processes from chemical/catalytic reactions at the cathode/electrolyte interface. Here, a new electron energy band diagrams concept is developed that includes the examination of the electrochemical- and ionization- potentials evolution upon batteries cycling.
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December 2024
TIME (Tübingen Institute for Medical Education), Medical Faculty Tuebingen, Eberhard Karls University Tuebingen, Hoppe-Seyler-Str. 3, Tuebingen, 72076, Germany.
Background: Effective healthcare delivery in today's diverse society necessitates healthcare providers' adeptness in navigating cultural and religious nuances in patient care. However, the integration of cultural competence training into medical education remains inadequate, particularly concerning the care of Muslim patients. In response, we introduce a novel educational intervention aimed at enhancing intercultural proficiency among medical students, emphasizing care for Muslim patients.
View Article and Find Full Text PDFInt J Mol Sci
December 2024
Renal Division, Department of Medicine, Faculty of Medicine, Medical Center, University of Freiburg, Hugstetter Strasse 55, 79106 Freiburg, Germany.
RAB11, a pivotal RabGTPase, regulates essential cellular processes such as endocytic recycling, exocytosis, and autophagy. The protein was implicated in various human diseases, including cancer, neurodegenerative disorders, viral infections, and podocytopathies. However, a small-molecular inhibitor is lacking.
View Article and Find Full Text PDFNanomaterials (Basel)
November 2024
Kurnakov Institute of General and Inorganic Chemistry, Russian Academy of Sciences, Leninsky Prospect, 31, Moscow 119991, Russia.
Cerium dioxide sols stabilised with L-malic acid were shown to exhibit significant antioxidant activity towards alkyl peroxyl radicals in the range of ligand:CeO molar ratios of 0.2-1 (0.2:1, 0.
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