Background: Tumor infiltrating B cells (TiBc) have not yet been investigated in detail. This may at least in part be due to technical difficulties. Here we describe a straightforward and reproducible method to isolate and culture TiBc from primary colorectal carcinomas (CRC).
Methods/results: TiBc cultures were generated by Epstein-Barr virus (EBV) immortalization. With this method, monoclonal TiBc cultures were obtained for 14/19 CRCs. As assessed by flow cytometry and ELISA, TiBc showed an activated immunophenotype (CD23(+), CD80(+)) and produced immunoglobulin (Ig; IgG secretion in 55% of the cultures). In functional in vitro analysis, most of the IgGs specifically bound to allogeneic CRC target cells. These data suggest that TiBc are antigen-experienced and thus may exhibit functionality in situ. Additionally, mini-cultures generated from 12 further CRCs revealed TiBc outgrowth exclusively in the presence of EBV.
Conclusion: In summary, this simple method provides a cellular tool and our data set the stage for analysing the bivalent role of TiBc; being antigen-presenting cells on the one hand and tumor-specific antibody producers on the other. Additionally, the generation of long-term TiBc cultures and their monoclonal Ig may serve to identify novel tumor-specific antigens.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3290587 | PMC |
| http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0032639 | PLOS |
Publication Analysis
Top Keywords
Similar Publications
Effect of an acute exercise on early responses of iron and iron regulatory proteins in young female basketball players.
BMC Sports Sci Med Rehabil
April 2022
Department of Biological Sciences, Faculty of Physical Culture in Gorzow Wielkopolski, Poznan University of Physical Education, Estkowskiego 13, 66-400, Gorzów, Wielkopolski, Poland.
Background: The accumulation of physiological stress and the presence of inflammation disturb iron management in athletes during intense training. However, little is known about the mechanisms regulating iron levels in athletes during training periods with low training loads. In the current study, we analyzed the effect of an acute exercise on early responses of iron and iron regulatory proteins at the end of such training periods.
View Article and Find Full Text PDFHemoglobin Concentration during Early Pregnancy as an Accurate Predictor of Anemia during Late Pregnancy.
Nutrients
February 2022
Department of Obstetrics and Gynecology, Hokkaido University Graduate School of Medicine, Sapporo 060-8638, Japan.
It is undetermined which blood variables related to iron storage during the first trimester of pregnancy could efficiently predict anemia occurring during the third trimester. Red blood cell count (RBC), hemoglobin concentration, hematocrit, ferritin, iron, and total iron binding capacity (TIBC) were assessed longitudinally during the first, second, and third trimesters of 231 healthy Japanese women. None of the patients had anemia in the first trimester and none used iron supplementation before the second trimester blood test.
View Article and Find Full Text PDFA novel cancer immunotherapy using tumor-infiltrating B cells in the APCmin/+ mouse model.
PLoS One
June 2021
Division of Cancer Cell Biology, Research Institute for Biomedical Sciences (RIBS), Tokyo University of Science, Noda, Japan.
Accumulating evidence has suggested a correlation of tumor infiltrating B cells (TiBcs) and a good prognosis of cancer diseases. In some cases, TiBcs appear to have experienced antigen stimulation since they have undergone class-switching and somatic hypermutation and formed tertiary lymphoid structures around tumors together with T cells. Assuming TiBcs include those that recognize some tumor antigens, we sought to investigate their possible usefulness for cell-mediated immunotherapies.
View Article and Find Full Text PDFThe protective role of Kombucha extract on the normal intestinal microflora, high-cholesterol diet caused hypercholesterolemia, and histological structures changes in New Zealand white rabbits.
Avicenna J Phytomed
January 2020
Department of Biology, Izeh Branch, Islamic Azad University, Izeh, Iran.
Objective: The aim of the current study was to investigate the effect of Kombucha extract (tea) on the normal intestinal microflora and histological structures in rabbits.
Materials And Methods: This study was a descriptive-analytical investigation. Thirty-two male New Zealand rabbits were randomly divided into 4 groups as follows: Normal diet (I), high-cholesterol diet (II), normal diet plus Kombucha extract (II), and high-cholesterol diet plus Kombucha extract (IV).
Comparison of camel, dog and the laboratory animals' sera with the fetal calf serum (FCS) for cultivation of .
J Parasit Dis
June 2020
Basic Sciences in Infectious Diseases Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
The best-known serum for spp. cultivation is the fetal calf serum (FCS), which is very expensive with ethical concerns. This study was conducted to compare various laboratory (BALB/c mice, rat, rabbit, hamster and guinea pig) and non-laboratory (dog and camel) animals' sera as a substitute for FCS in culture.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!