AI Article Synopsis
- BLAT (BLAST-Like Alignment Tool) helps users find genomic sequences that match submitted protein or DNA sequences, primarily within the same or closely related species.
- It was designed for faster alignment of large datasets, such as expressed sequence tags and random mouse genome reads to the human genome, and is available for free online or as a standalone program.
- The tool allows users to visualize results in the UCSC Genome Browser and includes protocols for identifying exon-intron locations, coding regions, and homologs in different species.
Article Abstract
The BLAST-Like Alignment Tool (BLAT) is used to find genomic sequences that match a protein or DNA sequence submitted by the user. BLAT is typically used for searching similar sequences within the same or closely related species. It was developed to align millions of expressed sequence tags and mouse whole-genome random reads to the human genome at a higher speed. It is freely available either on the Web or as a downloadable stand-alone program. BLAT search results provide a link for visualization in the University of California, Santa Cruz (UCSC) Genome Browser, where associated biological information may be obtained. Three example protocols are given: using an mRNA sequence to identify the exon-intron locations and associated gene in the genomic sequence of the same species, using a protein sequence to identify the coding regions in a genomic sequence and to search for gene family members in the same species, and using a protein sequence to find homologs in another species.
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Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4101998 | PMC |
| http://dx.doi.org/10.1002/0471250953.bi1008s37 | DOI Listing |
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