Epstein-Barr virus (EBV) latent infection transforms B lymphocytes into proliferating lymphoblastoid cell lines (LCLs). EBV latent infection membrane protein 1 (LMP1) is required for EBV-mediated B lymphocyte transformation, and LMP1-induced NF-κB activation is essential for LCL survival. To identify a novel inhibitor candidate for LMP1-induced NF-κB activation, crude ethanol extracts of medicinal plants were screened for the potential NF-κB inhibitory activity. Seventy percent ethanol extract of Chrysanthemum indicum Linne extract (CIE) strongly reduced LMP1-induced NF-κB activation. In addition, CIE inhibited LMP1-induced IKKα or IKKβ activation. Interestingly, CIE treatment rapidly reduced LCL viability without exhibiting any adverse effects on the viability of human foreskin fibroblasts (HFF), EBV negative Burkitt's lymphoma cell lines (BL41) or HeLa cells. Taken together, CIE has potent inhibitory effect on EBV LMP1-induced NF-κB activation and EBV-transformed LCL viability.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.fct.2012.02.034 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Decoding Critical Targets and Signaling Pathways in EBV-Mediated Diseases Using Large Language Models.
Viruses
October 2024
School of Public Health and Emergency Management, Southern University of Science and Technology, Shenzhen 518055, China.
Article Synopsis
- Epstein-Barr virus (EBV) is linked to several cancers, and understanding how it interacts with host cells is key to explaining its role in diseases like Burkitt lymphoma and nasopharyngeal carcinoma.
- This study used large language models to analyze over 36,000 scientific papers on EBV, finding that it primarily influences immune responses, cell growth, and apoptosis through specific protein interactions.
- The research uncovered potential diagnostic markers and highlighted LMP1's role in regulating glycolysis, offering a detailed overview of EBV's molecular interactions and their implications for therapy development.
Targeting the LMP1-ALIX axis in EBV nasopharyngeal carcinoma inhibits immunosuppressive small extracellular vesicle secretion and boosts anti-tumor immunity.
Cancer Commun (Lond)
December 2024
Department of Radiation and Medical Oncology, Hubei Key Laboratory of Tumor Biological Behaviors, Hubei Cancer Clinical Study Center, Zhongnan Hospital of Wuhan University, Wuhan, Hubei, P. R. China.
Article Synopsis
- Immunotherapy for nasopharyngeal carcinoma (NPC) is effective but has low response rates; the role of PD-L1 on small extracellular vesicles (sEVs) in tumor immunosuppression is a key focus of research.
- The study investigates how the Epstein-Barr virus protein LMP1 influences tumor immunity in NPC by analyzing T cell presence and sEV characteristics in both human tissues and mouse models.
- Results show that high LMP1 levels limit CD8 T cell infiltration and promote PD-L1 secretion through interactions with ALIX, suggesting a potential pathway for therapeutic intervention to enhance immune responses in NPC.
Lysine demethylase LSD1 is associated with stemness in EBV-positive B cell lymphoma.
Sci Rep
March 2024
Department of Health Sciences and Technology, Samsung Advanced Institute for Health Sciences and Technology, Sungkyunkwan University, Seoul, 06351, Korea.
EBV-infected lymphoma has a poor prognosis and various treatment strategies are being explored. Reports suggesting that B cell lymphoma can be induced by epigenetic regulation have piqued interest in studying mechanisms targeting epigenetic regulation. Here, we set out to identify an epigenetic regulator drug that acts synergistically with doxorubicin in EBV-positive lymphoma.
View Article and Find Full Text PDFDynamic bidirectional regulation of NLRC3 and gammaherpesviruses during viral latency in B lymphocytes.
J Med Virol
March 2024
Virus-Host Interactions Laboratory, Department of Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seoul, Republic of Korea.
Article Synopsis
- * During EBV infection, the viral protein LMP1 lowers NLRC3 levels, while simultaneously, NLRC3 works to degrade LMP1, creating a complex regulation system.
- * Research shows that in mice lacking NLRC3, there is increased viral latency after gammaherpesvirus infection, highlighting the importance of NLRC3 in controlling viral infections linked to potential tumorigenesis.
Growth Transformation of B Cells by Epstein-Barr Virus Requires Induction and Nucleolar Hypertrophy.
Microbiol Spectr
August 2023
Department of Virology, Fujita Health University School of Medicine, Toyoake, Japan.
Article Synopsis
- - The study investigates how Epstein-Barr virus (EBV) transforms primary B cells, which is the first step in developing posttransplant lymphoproliferative disorder (PTLD), highlighting increased nucleolar size and gene expression following infection.
- - Using RNA sequencing and knockout viruses, researchers found that EBV induces a specific gene responsible for nucleolar hypertrophy, which is vital for cell growth and proliferation, and that this process occurs rapidly after infection.
- - The research demonstrated that inhibiting IMPDH2 with mycophenolate mofetil (MMF) blocked B cell transformation by EBV, resulting in improved survival in a mouse model, suggesting MMF could be an effective treatment for suppressing PTLD.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!